Multiplexed Fourier Transform Infrared and Raman Imaging

Guillermo Quintás, Bayden R. Wood, Hugh J. Byrne, David Perez-Guaita

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review


Infrared (IR) and Raman spectroscopies are being increasingly employed for the label-free analysis of biochemical samples. Both are vibrational imaging techniques, but they provide complementary information about the chemical composition of the sample, and thus the integration of Raman and IR images leads to a comprehensive understanding of the samples. Here, we summarize the steps needed for performing multiplexed infrared and Raman imaging, identifying and overcoming the two main challenges: first, the technical difficulties caused by the incompatibilities of the techniques and, second, the necessity to extract the information from the large number of vibrational variables found in both IR and Raman spectra.

Original languageEnglish
Title of host publicationMultiplexed Imaging
Subtitle of host publicationMethods and Protocols
EditorsEli Zamir
Place of PublicationNew York NY United States
PublisherHumana Press
Number of pages14
ISBN (Electronic)9781071615935
ISBN (Print)9781071615928
Publication statusPublished - 31 Jul 2021

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Data fusion
  • Infrared
  • Label-free imaging
  • Multimodal imaging
  • Raman

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