Multiplexed Fourier Transform Infrared and Raman Imaging

Guillermo Quintás, Bayden R. Wood, Hugh J. Byrne, David Perez-Guaita

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

Infrared (IR) and Raman spectroscopies are being increasingly employed for the label-free analysis of biochemical samples. Both are vibrational imaging techniques, but they provide complementary information about the chemical composition of the sample, and thus the integration of Raman and IR images leads to a comprehensive understanding of the samples. Here, we summarize the steps needed for performing multiplexed infrared and Raman imaging, identifying and overcoming the two main challenges: first, the technical difficulties caused by the incompatibilities of the techniques and, second, the necessity to extract the information from the large number of vibrational variables found in both IR and Raman spectra.

Original languageEnglish
Title of host publicationMultiplexed Imaging
Subtitle of host publicationMethods and Protocols
EditorsEli Zamir
Place of PublicationNew York NY United States
PublisherHumana Press
Chapter19
Pages299-312
Number of pages14
Volume2350
ISBN (Electronic)9781071615935
ISBN (Print)9781071615928
DOIs
Publication statusPublished - 31 Jul 2021

Publication series

NameMethods in Molecular Biology
Volume2350
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Data fusion
  • Infrared
  • Label-free imaging
  • Multimodal imaging
  • Raman

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