TY - JOUR
T1 - Multiplex ligation-dependent probe amplification (MLPA) genotyping assay for mouse models of down syndrome
AU - Hewitt, Chelsea A
AU - Carmichael, Catherine L
AU - Wilkins, Ella J
AU - Cannon, Ping ZF
AU - Pritchard, Melanie April
AU - Scott, Hamish S
PY - 2007
Y1 - 2007
N2 - The Ts65Dn mouse is the most widely investigated segmentally trisomic mouse model of Down syndrome. Quantitative PCR based methods are the preferred way of detecting the trisomic segment for genotyping purposes. However, identification of a 1.5 fold difference in target DNA is at the limit of detection of most quantitative PCR based methods, and in practice this can lead to difficulties in assigning genotypes. We report a 100 accurate multiplex ligation-dependent probe amplification (MLPA) assay for genotyping the Ts65Dn mouse that is also applicable to all other segmentally trisomic mouse models of Down syndrome.
AB - The Ts65Dn mouse is the most widely investigated segmentally trisomic mouse model of Down syndrome. Quantitative PCR based methods are the preferred way of detecting the trisomic segment for genotyping purposes. However, identification of a 1.5 fold difference in target DNA is at the limit of detection of most quantitative PCR based methods, and in practice this can lead to difficulties in assigning genotypes. We report a 100 accurate multiplex ligation-dependent probe amplification (MLPA) assay for genotyping the Ts65Dn mouse that is also applicable to all other segmentally trisomic mouse models of Down syndrome.
UR - http://www.ncbi.nlm.nih.gov/pubmed/17485278
M3 - Article
SN - 1093-9946
VL - 12
SP - 3010
EP - 3016
JO - Frontiers in Bioscience
JF - Frontiers in Bioscience
ER -