Multiple semi-quantitative colorimetric assays in compact embeddable microfluidic cloth-based analytical device (muCAD) for effective point-of-care diagnostic

Azadeh Nilghaz, Saeedeh Bagherbaigi, Chee-Leong Lam, Seyed Mahdi Mousavi, Emma Paola Corcoles, Dedy H B Wicaksono

Research output: Contribution to journalArticleResearchpeer-review

32 Citations (Scopus)

Abstract

Cotton fabric is proposed as an alternative material
for low-cost point-of-care devices. Cotton fabrics are
vastly available, low cost and flexible. Simple wax patterning
method was applied to create hydrophilic channels in
cotton fabric. Three-dimensional (3D) colorimetric microfluidic
device was made by folding 2D pattern along certain
predefined lines. Three-dimensional devices show better
mixing uniformity between reagents and analyte across the
detection zones. On-chip colorimetric calibration is also proposed
by putting predefined serially diluted samples next to
the detection zones. Multiple assays can be integrated within
a small surface area by stacking layers of individual assay
device separated by a wax-impregnated fabric. We were able
to detect glucose, nitrite and protein having concentration as
low as 0.5 mM, 30 μM and 0.8 mg/mL, respectively, by bare
eyes. Results of the assays from an unknown analyte sample and precalibrated serially diluted sample standards were displayed
in a side-by-side configuration, and the interference
of each analyte on the other reaction zones was investigated.
These results are better than if the detection is merely taken
from the calibration curve without integrated standard calibration.
The mechanical durability, robustness and flexibility
of 3D microfluidic cloth-based analytical device (μCAD)
also make it easily embeddable to daily wearable product.
We demonstrated multiple single-step qualitative assays
using embedded 3D μCAD and propose a new concept of
“point-of-sampling diagnostic”.
Original languageEnglish
Pages (from-to)317 - 333
Number of pages17
JournalMicrofluidics and Nanofluidics
Volume19
Issue number2
DOIs
Publication statusPublished - 2015

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