Multiple Wolbachia strains provide comparative levels of protection against dengue virus infection in Aedes aegypti

Heather A. Flores, Jyotika Taneja de Bruyne, Tanya B. O’Donnell, Vu Tuyet Nhu, Nguyen Thi Giang, Huynh Thi Xuan Trang, Huynh Thi Thuy Van, Vo Thi Long, Le Thi Dui, Huynh Le Anh Huy, Huynh Thi Le Duyen, Nguyen Thi van Thuy, Nguyen Thanh Phong, Nguyen Van Vinh Chau, Duong Thi Hue Kien, Tran Thuy Vi, Bridget Wills, Scott L. O’Neill, Cameron P. Simmons, Lauren B. Carrington

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Abstract

The insect bacterium Wolbachia pipientis is being introgressed into Aedes aegypti populations as an intervention against the transmission of medically important arboviruses. Here we compare Ae. aegypti mosquitoes infected with wMelCS or wAlbB to the widely used wMel Wolbachia strain on an Australian nuclear genetic background for their susceptibility to infection by dengue virus (DENV) genotypes spanning all four serotypes. All Wolbachia-infected mosquitoes were more resistant to intrathoracic DENV challenge than their wildtype counterparts. Blocking of DENV replication was greatest by wMelCS. Conversely, wAlbB-infected mosquitoes were more susceptible to whole body infection than wMel and wMelCS. We extended these findings via mosquito oral feeding experiments, using viremic blood from 36 acute, hospitalised dengue cases in Vietnam, additionally including wMel and wildtype mosquitoes on a Vietnamese nuclear genetic background. As above, wAlbB was less effective at blocking DENV replication in the abdomen compared to wMel and wMelCS. The transmission potential of all Wolbachia-infected mosquito lines (measured by the presence/ absence of infectious DENV in mosquito saliva) after 14 days, was significantly reduced compared to their wildtype counterparts, and lowest for wMelCS and wAlbB. These data support the use of wAlbB and wMelCS strains for introgression field trials and the biocontrol of DENV transmission. Furthermore, despite observing significant differences in transmission potential between wildtype mosquitoes from Australia and Vietnam, no difference was observed between wMel-infected mosquitoes from each background suggesting that Wolbachia may override any underlying variation in DENV transmission potential.

Original languageEnglish
Article numbere1008433
Number of pages17
JournalPLoS Pathogens
Volume16
Issue number4
DOIs
Publication statusPublished - Apr 2020

Cite this

Flores, H. A., de Bruyne, J. T., O’Donnell, T. B., Nhu, V. T., Giang, N. T., Trang, H. T. X., Van, H. T. T., Long, V. T., Dui, L. T., Huy, H. L. A., Le Duyen, H. T., van Thuy, N. T., Phong, N. T., Chau, N. V. V., Kien, D. T. H., Vi, T. T., Wills, B., O’Neill, S. L., Simmons, C. P., & Carrington, L. B. (2020). Multiple Wolbachia strains provide comparative levels of protection against dengue virus infection in Aedes aegypti. PLoS Pathogens, 16(4), [e1008433]. https://doi.org/10.1371/journal.ppat.1008433