Multiphoton imaging reveals a new leukocyte recruitment paradigm in the glomerulus

Sapna Devi, Anqi Li, Clare Louise Westhorpe, Camden Yeung-Wah Lo, Latasha Abeynaike, Sarah Louise Snelgrove, Pamela Heidi Hall, Joshua Ooi, Christopher Graeme Sobey, Arthur Richard Kitching, Michael John Hickey

Research output: Contribution to journalArticleResearchpeer-review

Abstract

In contrast with many capillary beds, the glomerulus readily supports leukocyte recruitment. However, little is known regarding the actions of leukocytes following their recruitment to glomeruli. We used multiphoton confocal microscopy to examine leukocyte behavior in the glomerular microvasculature. In normal glomeruli, neutrophils and monocytes were retained in capillaries for several minutes, remaining static or migrating intravascularly. Induction of glomerular inflammation resulted in an increase in the duration of retention of static and migratory leukocytes. In response to immune complex deposition, both static and migratory neutrophils generated oxidants in inflamed glomeruli via a Mac-1-dependent mechanism. Our results describe a new paradigm for glomerular inflammation, suggesting that the major effect of acute inflammation is to increase the duration of leukocyte retention in the glomerulus. Moreover, these findings describe a previously unknown form of multicellular intravascular patrolling that involves both monocytes and neutrophils, which may underlie the susceptibility of the glomerulus to inflammation.
Original languageEnglish
Pages (from-to)107 - 112
Number of pages6
JournalNature Medicine
Volume19
Issue number1
DOIs
Publication statusPublished - 2013

Cite this

Devi, Sapna ; Li, Anqi ; Westhorpe, Clare Louise ; Lo, Camden Yeung-Wah ; Abeynaike, Latasha ; Snelgrove, Sarah Louise ; Hall, Pamela Heidi ; Ooi, Joshua ; Sobey, Christopher Graeme ; Kitching, Arthur Richard ; Hickey, Michael John. / Multiphoton imaging reveals a new leukocyte recruitment paradigm in the glomerulus. In: Nature Medicine. 2013 ; Vol. 19, No. 1. pp. 107 - 112.
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abstract = "In contrast with many capillary beds, the glomerulus readily supports leukocyte recruitment. However, little is known regarding the actions of leukocytes following their recruitment to glomeruli. We used multiphoton confocal microscopy to examine leukocyte behavior in the glomerular microvasculature. In normal glomeruli, neutrophils and monocytes were retained in capillaries for several minutes, remaining static or migrating intravascularly. Induction of glomerular inflammation resulted in an increase in the duration of retention of static and migratory leukocytes. In response to immune complex deposition, both static and migratory neutrophils generated oxidants in inflamed glomeruli via a Mac-1-dependent mechanism. Our results describe a new paradigm for glomerular inflammation, suggesting that the major effect of acute inflammation is to increase the duration of leukocyte retention in the glomerulus. Moreover, these findings describe a previously unknown form of multicellular intravascular patrolling that involves both monocytes and neutrophils, which may underlie the susceptibility of the glomerulus to inflammation.",
author = "Sapna Devi and Anqi Li and Westhorpe, {Clare Louise} and Lo, {Camden Yeung-Wah} and Latasha Abeynaike and Snelgrove, {Sarah Louise} and Hall, {Pamela Heidi} and Joshua Ooi and Sobey, {Christopher Graeme} and Kitching, {Arthur Richard} and Hickey, {Michael John}",
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Multiphoton imaging reveals a new leukocyte recruitment paradigm in the glomerulus. / Devi, Sapna; Li, Anqi; Westhorpe, Clare Louise; Lo, Camden Yeung-Wah; Abeynaike, Latasha; Snelgrove, Sarah Louise; Hall, Pamela Heidi; Ooi, Joshua; Sobey, Christopher Graeme; Kitching, Arthur Richard; Hickey, Michael John.

In: Nature Medicine, Vol. 19, No. 1, 2013, p. 107 - 112.

Research output: Contribution to journalArticleResearchpeer-review

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