Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 107 - 112 |
| Number of pages | 6 |
| Journal | Nature Medicine |
| Volume | 19 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2013 |
Cite this
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Multiphoton imaging reveals a new leukocyte recruitment paradigm in the glomerulus. / Devi, Sapna; Li, Anqi; Westhorpe, Clare Louise; Lo, Camden Yeung-Wah; Abeynaike, Latasha; Snelgrove, Sarah Louise; Hall, Pamela Heidi; Ooi, Joshua; Sobey, Christopher Graeme; Kitching, Arthur Richard; Hickey, Michael John.
In: Nature Medicine, Vol. 19, No. 1, 2013, p. 107 - 112.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Multiphoton imaging reveals a new leukocyte recruitment paradigm in the glomerulus
AU - Devi, Sapna
AU - Li, Anqi
AU - Westhorpe, Clare Louise
AU - Lo, Camden Yeung-Wah
AU - Abeynaike, Latasha
AU - Snelgrove, Sarah Louise
AU - Hall, Pamela Heidi
AU - Ooi, Joshua
AU - Sobey, Christopher Graeme
AU - Kitching, Arthur Richard
AU - Hickey, Michael John
PY - 2013
Y1 - 2013
N2 - In contrast with many capillary beds, the glomerulus readily supports leukocyte recruitment. However, little is known regarding the actions of leukocytes following their recruitment to glomeruli. We used multiphoton confocal microscopy to examine leukocyte behavior in the glomerular microvasculature. In normal glomeruli, neutrophils and monocytes were retained in capillaries for several minutes, remaining static or migrating intravascularly. Induction of glomerular inflammation resulted in an increase in the duration of retention of static and migratory leukocytes. In response to immune complex deposition, both static and migratory neutrophils generated oxidants in inflamed glomeruli via a Mac-1-dependent mechanism. Our results describe a new paradigm for glomerular inflammation, suggesting that the major effect of acute inflammation is to increase the duration of leukocyte retention in the glomerulus. Moreover, these findings describe a previously unknown form of multicellular intravascular patrolling that involves both monocytes and neutrophils, which may underlie the susceptibility of the glomerulus to inflammation.
AB - In contrast with many capillary beds, the glomerulus readily supports leukocyte recruitment. However, little is known regarding the actions of leukocytes following their recruitment to glomeruli. We used multiphoton confocal microscopy to examine leukocyte behavior in the glomerular microvasculature. In normal glomeruli, neutrophils and monocytes were retained in capillaries for several minutes, remaining static or migrating intravascularly. Induction of glomerular inflammation resulted in an increase in the duration of retention of static and migratory leukocytes. In response to immune complex deposition, both static and migratory neutrophils generated oxidants in inflamed glomeruli via a Mac-1-dependent mechanism. Our results describe a new paradigm for glomerular inflammation, suggesting that the major effect of acute inflammation is to increase the duration of leukocyte retention in the glomerulus. Moreover, these findings describe a previously unknown form of multicellular intravascular patrolling that involves both monocytes and neutrophils, which may underlie the susceptibility of the glomerulus to inflammation.
UR - http://www.nature.com/nm/journal/v19/n1/pdf/nm.3024.pdf
U2 - 10.1038/nm.3024
DO - 10.1038/nm.3024
M3 - Article
VL - 19
SP - 107
EP - 112
JO - Nature Medicine
JF - Nature Medicine
SN - 1078-8956
IS - 1
ER -