Multiphoton fluorescence microscopy of the live kidney in health and disease

David Small, Washington Y Sanchez, Sandrine Roy, Michael John Hickey, Glenda C Gobe

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The structural and functional heterogeneity of the kidney ensures a diversity of response in health and disease. Multiphoton microscopy has improved our understanding of kidney physiology and pathophysiology by enabling the visualization of the living kidney in comparison with the static view of previous technologies. The use of multiphoton microscopy with rodent models in conjunction with endogenous fluorescence and exogenous infused dyes permits the measurement of renal processes, such as glomerular permeability, juxtaglomerular apparatus function, tubulointerstitial function, tubulovascular interactions, vascular flow rate, and the intrarenal renin-angiotensin-aldosterone system. Subcellular processes, including mitochondrial dynamics, reactive oxygen species production, cytosolic ion concentrations, and death processes apoptosis and necrosis, can also be measured by multiphoton microscopy. This has allowed valuable insight into the pathophysiology of diabetic nephropathy, renal ischemia-reperfusion injury, hypertensive nephropathy, as well as inflammatory responses of the kidney. The current review presents an overview of multiphoton microscopy with a focus on techniques for imaging the kidney and gives examples of instances where multiphoton microscopy has been utilized to study renal pathophysiology in the living kidney. With continued advancements in the field of biological optics and increased adoption in experimental nephrology, multiphoton microscopy will undoubtedly continue to create new paradigms in kidney disease.
Original languageEnglish
Pages (from-to)1 - 13
Number of pages13
JournalJournal of Biomedical Optics
Volume19
Issue number2 (Art. ID: e02090)
DOIs
Publication statusPublished - 2014

Cite this

Small, David ; Sanchez, Washington Y ; Roy, Sandrine ; Hickey, Michael John ; Gobe, Glenda C. / Multiphoton fluorescence microscopy of the live kidney in health and disease. In: Journal of Biomedical Optics. 2014 ; Vol. 19, No. 2 (Art. ID: e02090). pp. 1 - 13.
@article{8f294a2a1a764613b535231eab882b9f,
title = "Multiphoton fluorescence microscopy of the live kidney in health and disease",
abstract = "The structural and functional heterogeneity of the kidney ensures a diversity of response in health and disease. Multiphoton microscopy has improved our understanding of kidney physiology and pathophysiology by enabling the visualization of the living kidney in comparison with the static view of previous technologies. The use of multiphoton microscopy with rodent models in conjunction with endogenous fluorescence and exogenous infused dyes permits the measurement of renal processes, such as glomerular permeability, juxtaglomerular apparatus function, tubulointerstitial function, tubulovascular interactions, vascular flow rate, and the intrarenal renin-angiotensin-aldosterone system. Subcellular processes, including mitochondrial dynamics, reactive oxygen species production, cytosolic ion concentrations, and death processes apoptosis and necrosis, can also be measured by multiphoton microscopy. This has allowed valuable insight into the pathophysiology of diabetic nephropathy, renal ischemia-reperfusion injury, hypertensive nephropathy, as well as inflammatory responses of the kidney. The current review presents an overview of multiphoton microscopy with a focus on techniques for imaging the kidney and gives examples of instances where multiphoton microscopy has been utilized to study renal pathophysiology in the living kidney. With continued advancements in the field of biological optics and increased adoption in experimental nephrology, multiphoton microscopy will undoubtedly continue to create new paradigms in kidney disease.",
author = "David Small and Sanchez, {Washington Y} and Sandrine Roy and Hickey, {Michael John} and Gobe, {Glenda C}",
year = "2014",
doi = "10.1117/1.JBO.19.2.020901",
language = "English",
volume = "19",
pages = "1 -- 13",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "SPIE",
number = "2 (Art. ID: e02090)",

}

Multiphoton fluorescence microscopy of the live kidney in health and disease. / Small, David; Sanchez, Washington Y; Roy, Sandrine; Hickey, Michael John; Gobe, Glenda C.

In: Journal of Biomedical Optics, Vol. 19, No. 2 (Art. ID: e02090), 2014, p. 1 - 13.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Multiphoton fluorescence microscopy of the live kidney in health and disease

AU - Small, David

AU - Sanchez, Washington Y

AU - Roy, Sandrine

AU - Hickey, Michael John

AU - Gobe, Glenda C

PY - 2014

Y1 - 2014

N2 - The structural and functional heterogeneity of the kidney ensures a diversity of response in health and disease. Multiphoton microscopy has improved our understanding of kidney physiology and pathophysiology by enabling the visualization of the living kidney in comparison with the static view of previous technologies. The use of multiphoton microscopy with rodent models in conjunction with endogenous fluorescence and exogenous infused dyes permits the measurement of renal processes, such as glomerular permeability, juxtaglomerular apparatus function, tubulointerstitial function, tubulovascular interactions, vascular flow rate, and the intrarenal renin-angiotensin-aldosterone system. Subcellular processes, including mitochondrial dynamics, reactive oxygen species production, cytosolic ion concentrations, and death processes apoptosis and necrosis, can also be measured by multiphoton microscopy. This has allowed valuable insight into the pathophysiology of diabetic nephropathy, renal ischemia-reperfusion injury, hypertensive nephropathy, as well as inflammatory responses of the kidney. The current review presents an overview of multiphoton microscopy with a focus on techniques for imaging the kidney and gives examples of instances where multiphoton microscopy has been utilized to study renal pathophysiology in the living kidney. With continued advancements in the field of biological optics and increased adoption in experimental nephrology, multiphoton microscopy will undoubtedly continue to create new paradigms in kidney disease.

AB - The structural and functional heterogeneity of the kidney ensures a diversity of response in health and disease. Multiphoton microscopy has improved our understanding of kidney physiology and pathophysiology by enabling the visualization of the living kidney in comparison with the static view of previous technologies. The use of multiphoton microscopy with rodent models in conjunction with endogenous fluorescence and exogenous infused dyes permits the measurement of renal processes, such as glomerular permeability, juxtaglomerular apparatus function, tubulointerstitial function, tubulovascular interactions, vascular flow rate, and the intrarenal renin-angiotensin-aldosterone system. Subcellular processes, including mitochondrial dynamics, reactive oxygen species production, cytosolic ion concentrations, and death processes apoptosis and necrosis, can also be measured by multiphoton microscopy. This has allowed valuable insight into the pathophysiology of diabetic nephropathy, renal ischemia-reperfusion injury, hypertensive nephropathy, as well as inflammatory responses of the kidney. The current review presents an overview of multiphoton microscopy with a focus on techniques for imaging the kidney and gives examples of instances where multiphoton microscopy has been utilized to study renal pathophysiology in the living kidney. With continued advancements in the field of biological optics and increased adoption in experimental nephrology, multiphoton microscopy will undoubtedly continue to create new paradigms in kidney disease.

UR - http://www.ncbi.nlm.nih.gov/pubmed/24525825

U2 - 10.1117/1.JBO.19.2.020901

DO - 10.1117/1.JBO.19.2.020901

M3 - Article

VL - 19

SP - 1

EP - 13

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 2 (Art. ID: e02090)

ER -