Multiparameter toxicity screening on a chip

Effects of UV radiation and titanium dioxide nanoparticles on HaCaT cells

Scott McCormick, Louise E. Smith, Amy M. Holmes, Ziqiu Tong, Enzo Lombi, Nicolas H. Voelcker, Craig Priest

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Microfluidic screening is gaining attention as an efficient method for evaluating nanomaterial toxicity. Here, we consider a multiparameter treatment where nanomaterials interact with cells in the presence of a secondary exposure (UV radiation). The microfluidic device contains channels that permit immobilization of HaCaT cells (human skin cell line), delivery of titanium dioxide nanoparticles (TNPs), and exposure to a known dose of UV radiation. The effect of single-parameter exposures (UV or TNP) was first studied as a benchmark, and then multiparameter toxicity (UV and TNP) at different concentrations was explored. The results demonstrate a concentration-dependent protective effect of TNP when exposed to UV irradiation.

Original languageEnglish
Article number044112
Number of pages8
JournalBiomicrofluidics
Volume13
Issue number4
DOIs
Publication statusPublished - 1 Jul 2019

Cite this

McCormick, Scott ; Smith, Louise E. ; Holmes, Amy M. ; Tong, Ziqiu ; Lombi, Enzo ; Voelcker, Nicolas H. ; Priest, Craig. / Multiparameter toxicity screening on a chip : Effects of UV radiation and titanium dioxide nanoparticles on HaCaT cells. In: Biomicrofluidics. 2019 ; Vol. 13, No. 4.
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Multiparameter toxicity screening on a chip : Effects of UV radiation and titanium dioxide nanoparticles on HaCaT cells. / McCormick, Scott; Smith, Louise E.; Holmes, Amy M.; Tong, Ziqiu; Lombi, Enzo; Voelcker, Nicolas H.; Priest, Craig.

In: Biomicrofluidics, Vol. 13, No. 4, 044112, 01.07.2019.

Research output: Contribution to journalArticleResearchpeer-review

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