Multiparameter toxicity screening on a chip: Effects of UV radiation and titanium dioxide nanoparticles on HaCaT cells

Scott McCormick, Louise E. Smith, Amy M. Holmes, Ziqiu Tong, Enzo Lombi, Nicolas H. Voelcker, Craig Priest

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Microfluidic screening is gaining attention as an efficient method for evaluating nanomaterial toxicity. Here, we consider a multiparameter treatment where nanomaterials interact with cells in the presence of a secondary exposure (UV radiation). The microfluidic device contains channels that permit immobilization of HaCaT cells (human skin cell line), delivery of titanium dioxide nanoparticles (TNPs), and exposure to a known dose of UV radiation. The effect of single-parameter exposures (UV or TNP) was first studied as a benchmark, and then multiparameter toxicity (UV and TNP) at different concentrations was explored. The results demonstrate a concentration-dependent protective effect of TNP when exposed to UV irradiation.

Original languageEnglish
Article number044112
Number of pages8
JournalBiomicrofluidics
Volume13
Issue number4
DOIs
Publication statusPublished - 1 Jul 2019

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