@inbook{f014090b23034bb998bfda99feaef0f2,
title = "Multiparameter Fluorescence-Activated Cell Sorting of Human Lymphatic Endothelial Cells",
abstract = "Multiparameter fluorescence-activated cell sorting (FACS) procedure separates target cells from a total population of cells by using specific signatures that the target cell expresses on their cell surface. For human lymphatic endothelial cells (LECs) this relates to cell surface expression of the CD34LowCD31HighVEGFR-3HighPodoplaninHigh profile that permits their separation from blood vascular endothelial cells and other cells likely to be present in the digested tissue sample. In addition, FACS allows the evaluation of LEC size, volume, granularity, and purity at the time of sorting.",
keywords = "Cell culture, Cell surface markers, FACS, Lymphatic endothelial cell",
author = "Theresa Connor and Nerida Sleebs and Zerina Lokmic-Tomkins",
note = "Funding Information: The authors acknowledge the Baker Foundation and the Royal Children{\textquoteright}s Foundation “Women in Science Fellowship” support of Zerina Lokmic-Tomkins and the NHMRC grant AP 1085109 supporting lymphatic malformations flow cytometry and FACS methodology work. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2217-9_3",
language = "English",
isbn = "9781071622162",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "47--59",
editor = "Fiedler, {Lorna R.} and Caroline Pellet-Many",
booktitle = "VEGF Signaling",
address = "United States of America",
edition = "2nd",
}