Multifunctional poly(2-oxazoline) nanoparticles for biological applications

Kristian Kempe, Antje Vollrath, Hendrik W. Schaefer, Tobias G. Poehlmann, Christoph Biskup, Richard Hoogenboom, Stephanie Hornig, Ulrich S. Schubert

Research output: Contribution to journalArticleResearchpeer-review

57 Citations (Scopus)

Abstract

New multifunctional copoly(2-oxazoline) nanoparticles were prepared for cell studies. The polymer contains double-bond side chains as potential reaction sites for "thio"-click reactions as well as a fluorescein label covalently bound to the polymer backbone. Using the nanoprecipitation technique, spherical nanoparticles of 200-800 nm were obtained. Confocal laser scanning microscopy measurements revealed the cellular uptake of the nanoparticles. Multifunctional copoly(2-oxazoline) nanoparticles were prepared that were taken up by cells as revealed by confocal laser scanning microscopy measurements. The polymer contains double bond side chains as potential reaction sites for "thio"-click reactions as well as a fluorescein label covalently bound to the polymer backbone. Using the nanoprecipitation technique, spherical nanoparticles of 200-800 nm were obtained. Confocal laser scanning microscopy measurements and flow cytometry shows uptake of the particles into the cells without any toxic effect.

Original languageEnglish
Pages (from-to)1869-1873
Number of pages5
JournalMacromolecular Rapid Communications
Volume31
Issue number21
DOIs
Publication statusPublished - 1 Nov 2010
Externally publishedYes

Keywords

  • Copolymers
  • Fluorescence microscopy
  • Living polymerization
  • Nanoparticles
  • Nanoprecipitation
  • Poly(2-oxazoline)

Cite this

Kempe, K., Vollrath, A., Schaefer, H. W., Poehlmann, T. G., Biskup, C., Hoogenboom, R., Hornig, S., & Schubert, U. S. (2010). Multifunctional poly(2-oxazoline) nanoparticles for biological applications. Macromolecular Rapid Communications, 31(21), 1869-1873. https://doi.org/10.1002/marc.201000283