Multifunctional poly(2-oxazoline) nanoparticles for biological applications

Kristian Kempe; Antje Vollrath; Hendrik W. Schaefer; Tobias G. Poehlmann; Christoph Biskup; Richard Hoogenboom; Stephanie Hornig; Ulrich S. Schubert

doi:10.1002/marc.201000283

Multifunctional poly(2-oxazoline) nanoparticles for biological applications

Kristian Kempe, Antje Vollrath, Hendrik W. Schaefer, Tobias G. Poehlmann, Christoph Biskup, Richard Hoogenboom, Stephanie Hornig, Ulrich S. Schubert

Research output: Contribution to journal › Article › Research › peer-review

67 Citations (Scopus)

Abstract

New multifunctional copoly(2-oxazoline) nanoparticles were prepared for cell studies. The polymer contains double-bond side chains as potential reaction sites for "thio"-click reactions as well as a fluorescein label covalently bound to the polymer backbone. Using the nanoprecipitation technique, spherical nanoparticles of 200-800 nm were obtained. Confocal laser scanning microscopy measurements revealed the cellular uptake of the nanoparticles. Multifunctional copoly(2-oxazoline) nanoparticles were prepared that were taken up by cells as revealed by confocal laser scanning microscopy measurements. The polymer contains double bond side chains as potential reaction sites for "thio"-click reactions as well as a fluorescein label covalently bound to the polymer backbone. Using the nanoprecipitation technique, spherical nanoparticles of 200-800 nm were obtained. Confocal laser scanning microscopy measurements and flow cytometry shows uptake of the particles into the cells without any toxic effect.

Original language	English
Pages (from-to)	1869-1873
Number of pages	5
Journal	Macromolecular Rapid Communications
Volume	31
Issue number	21
DOIs	https://doi.org/10.1002/marc.201000283
Publication status	Published - 1 Nov 2010
Externally published	Yes

Keywords

Copolymers
Fluorescence microscopy
Living polymerization
Nanoparticles
Nanoprecipitation
Poly(2-oxazoline)

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10.1002/marc.201000283

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abstract = "New multifunctional copoly(2-oxazoline) nanoparticles were prepared for cell studies. The polymer contains double-bond side chains as potential reaction sites for {"}thio{"}-click reactions as well as a fluorescein label covalently bound to the polymer backbone. Using the nanoprecipitation technique, spherical nanoparticles of 200-800 nm were obtained. Confocal laser scanning microscopy measurements revealed the cellular uptake of the nanoparticles. Multifunctional copoly(2-oxazoline) nanoparticles were prepared that were taken up by cells as revealed by confocal laser scanning microscopy measurements. The polymer contains double bond side chains as potential reaction sites for {"}thio{"}-click reactions as well as a fluorescein label covalently bound to the polymer backbone. Using the nanoprecipitation technique, spherical nanoparticles of 200-800 nm were obtained. Confocal laser scanning microscopy measurements and flow cytometry shows uptake of the particles into the cells without any toxic effect.",

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AU - Biskup, Christoph

AU - Hoogenboom, Richard

AU - Hornig, Stephanie

AU - Schubert, Ulrich S.

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AB - New multifunctional copoly(2-oxazoline) nanoparticles were prepared for cell studies. The polymer contains double-bond side chains as potential reaction sites for "thio"-click reactions as well as a fluorescein label covalently bound to the polymer backbone. Using the nanoprecipitation technique, spherical nanoparticles of 200-800 nm were obtained. Confocal laser scanning microscopy measurements revealed the cellular uptake of the nanoparticles. Multifunctional copoly(2-oxazoline) nanoparticles were prepared that were taken up by cells as revealed by confocal laser scanning microscopy measurements. The polymer contains double bond side chains as potential reaction sites for "thio"-click reactions as well as a fluorescein label covalently bound to the polymer backbone. Using the nanoprecipitation technique, spherical nanoparticles of 200-800 nm were obtained. Confocal laser scanning microscopy measurements and flow cytometry shows uptake of the particles into the cells without any toxic effect.

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KW - Fluorescence microscopy

KW - Living polymerization

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KW - Nanoprecipitation

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Multifunctional poly(2-oxazoline) nanoparticles for biological applications

Abstract

Keywords

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