'Multicopy multivalent' glycopolymer-stabilized gold nanoparticles as potential synthetic cancer vaccines

Alison L. Parry, Natasha A. Clemson, James Ellis, Stefan S. R. Bernhard, Benjamin G. Davis, Neil R. Cameron

Research output: Contribution to journalArticleResearchpeer-review

147 Citations (Scopus)

Abstract

Mucin-related carbohydrates are overexpressed on the surface of cancer cells, providing a disease-specific target for cancer immunotherapy. Here, we describe the design and construction of peptide-free multivalent glycosylated nanoscale constructs as potential synthetic cancer vaccines that generate significant titers of antibodies selective for aberrant mucin glycans. A polymerizable version of the Tn-antigen glycan was prepared and converted into well-defined glycopolymers by Reversible Addition-Fragmentation chain Transfer (RAFT) polymerization. The polymers were then conjugated to gold nanoparticles, yielding 'multicopy-multivalent' nanoscale glycoconjugates. Immunological studies indicated that these nanomaterials generated strong and long-lasting production of antibodies that are selective to the Tn-antigen glycan and cross-reactive toward mucin proteins displaying Tn. The results demonstrate proof-of-concept of a simple and modular approach toward synthetic anticancer vaccines based on multivalent glycosylated nanomaterials without the need for a typical vaccine protein component. 

Original languageEnglish
Pages (from-to)9362-9365
Number of pages4
JournalJournal of the American Chemical Society
Volume135
Issue number25
DOIs
Publication statusPublished - 13 Jun 2013
Externally publishedYes

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