Mucosal and systemic immunogenicity of a recombinant, non-ADP-ribosylating pertussis toxin: effects of formaldehyde treatment

I. Cropley, G. Douce, M. Roberts, S. Chatfield, M. Pizza, R. Rappuoli, G. Dougan

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Abstract

The effect of formaldehyde treatment on the mucosal and systemic immunogenicity of the geneticallydetoxified pertussis toxin ( PT-9K 129G) was investigated. Groups of BALB c were immunized intranasally (i.n.) or subcutaneously (s.c.) with untreated, lightly formaldehyde treated (LFT) or heavily formaldehyde treated (HFT) recombinant pertussis toxin (PT) mutant, PT-9K 129G. Intranasal immunization with native PT-9K 129G induced significant levels of anti-toxin antibodies in serum and IgA anti-toxin responses in nasal and lung lavages of these mice. Similar local and systemic responses were observed following intransal immunization with LFT toxin. However, i.n. immunization with HFT toxin failed to induce a local IgA response and elicited a much diminished anti-toxin response in the serum. In contrast, the total antibody response following s.c. immunization was not significantly affected. In addition, i.n. immunization with native PT-9K 129G induced low but detectable levels of toxin neutralizing antibodies in the serum. These results show that native PT-9K 129G protein acts as a mucosal immunogen in mice and that this activity is greatly diminished by HFT of the protein.

Original languageEnglish
Pages (from-to)1643-1648
Number of pages6
JournalVaccine
Volume13
Issue number17
DOIs
Publication statusPublished - 1 Jan 1995
Externally publishedYes

Keywords

  • formaldehyde
  • mucosal immunization
  • Pertussis toxin

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