Mouse redox histology using genetically encoded probes

Yuuta Fujikawa, Leticia P. Roma, Mirko C. Sobotta, Adam J. Rose, Mauricio Berriel Diaz, Giuseppe Locatelli, Michael O. Breckwoldt, Thomas Misgeld, Martin Kerschensteiner, Stephan Herzig, Karin Müller-Decker, Tobias P. Dick

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50 Citations (Scopus)


Mapping the in vivo distribution of endogenous oxidants in animal tissues is of substantial biomedical interest. Numerous health-related factors, including diet, physical activity, infection, aging, toxins, or pharmacological intervention, may cause redox changes. Tools are needed to pinpoint redox state changes to particular organs, tissues, cell types, and subcellular organelles. We describe a procedure that preserves the in vivo redox state of genetically encoded redox biosensors within histological tissue sections, thus providing "redox maps" for any tissue and comparison of interest. We demonstrate the utility of the technique by visualizing endogenous redox differences and changes in the context of tumor growth, inflammation, embryonic development, and nutrient starvation.

Original languageEnglish
Article numberRS1
Number of pages10
JournalScience Signaling
Issue number419
Publication statusPublished - 15 Mar 2016
Externally publishedYes

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