Mouse hematopoietic stem cell modification and labelling by transduction and tracking posttransplantation

Benjamin Cao, Songhui Li, Claire Pritchard, Brenda Williams, Susan K. Nilsson

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

The tracking of the hematopoietic potential of genetically manipulated fluorescent hematopoietic stem cells (HSC) in the bone marrow (BM) allows the assessment of regulatory processes involved in the re-establishment of hematopoiesis posttransplant. Herein, we describe the means to assess the consequence of expressing specific genes in HSC on their engraftment potential posttransplant.

Original languageEnglish
Title of host publicationMouse Cell Culture
Place of PublicationNew York, NY
PublisherHumana Press
Chapter9
Pages129-142
Number of pages14
ISBN (Electronic)9781493990863
ISBN (Print)9781493990856
DOIs
Publication statusPublished - 1 Jan 2019

Publication series

NameMethods in Molecular Biology
Volume1940
ISSN (Print)1064-3745

Keywords

  • Bone marrow transplantation
  • Hematopoietic stem cells
  • Lentiviral transduction

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