Morphology and function of the lamb ileum following preterm birth

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background: For infants born moderately/late preterm (32-37 weeks of gestation), immaturity of the intestine has the potential to impact both short- and long-term gastrointestinal function. The aim of this study conducted in sheep was to compare the morphology and smooth muscle contractility of the ileum in term and late preterm lambs. Materials and methods: Lambs delivered preterm (132 days gestation; n = 7) or term (147 days gestation; n = 9) were milk-fed after birth and euthanased at 2 days of age. A segment of distal ileum was collected for analysis of the length and cellular composition of the villi and crypts, smooth muscle width and contractility, and mRNA expression of the cell markers Ki67, lysozyme, mucin 2, synaptophysin, chromogranin A, olfactomedin 4, axis inhibition protein 2, and leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5). results: There was no difference in the proportion of inflammatory, proliferating, apoptotic, enterocyte, or goblet cells between groups, but preterm lambs exhibited a significant upregulation of the stem cell marker LGR5 (p = 0.01). Absolute villus height (term: 1,032 ± 147 μm, preterm: 651 ± 52 μm; p < 0.0001) and crypt depth (term: 153 ± 11 μm, preterm: 133 ± 17 μm; p = 0.01) were significantly shorter in the preterm ileums, with a trend (p = 0.06) for a reduction in muscularis externa width. There was no difference between groups in the contractile response to acetylcholine, but peak contractility in response to bradykinin (p = 0.02) and angiotensin II (p = 0.03) was significantly greater in the preterm lambs. conclusion: Findings demonstrate that the crypt-villus units are shorter in the ileum of late preterm offspring, but functionally mature with an equivalent cellular composition and normal contractile response to acetylcholine compared with term offspring. The exaggerated contractility to inflammatory mediators evident in the preterm ileum, however, may be of concern.

Original languageEnglish
Article number8
Number of pages11
JournalFrontiers in Pediatrics
Volume6
DOIs
Publication statusPublished - 29 Jan 2018

Keywords

  • Development
  • Gastrointestinal tract
  • Ileum
  • Inflammatory bowel disease
  • Preterm birth
  • Sheep
  • Small intestine

Cite this

@article{1fc8ddf4d76841e1bf9b31addd4fa1f8,
title = "Morphology and function of the lamb ileum following preterm birth",
abstract = "Background: For infants born moderately/late preterm (32-37 weeks of gestation), immaturity of the intestine has the potential to impact both short- and long-term gastrointestinal function. The aim of this study conducted in sheep was to compare the morphology and smooth muscle contractility of the ileum in term and late preterm lambs. Materials and methods: Lambs delivered preterm (132 days gestation; n = 7) or term (147 days gestation; n = 9) were milk-fed after birth and euthanased at 2 days of age. A segment of distal ileum was collected for analysis of the length and cellular composition of the villi and crypts, smooth muscle width and contractility, and mRNA expression of the cell markers Ki67, lysozyme, mucin 2, synaptophysin, chromogranin A, olfactomedin 4, axis inhibition protein 2, and leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5). results: There was no difference in the proportion of inflammatory, proliferating, apoptotic, enterocyte, or goblet cells between groups, but preterm lambs exhibited a significant upregulation of the stem cell marker LGR5 (p = 0.01). Absolute villus height (term: 1,032 ± 147 μm, preterm: 651 ± 52 μm; p < 0.0001) and crypt depth (term: 153 ± 11 μm, preterm: 133 ± 17 μm; p = 0.01) were significantly shorter in the preterm ileums, with a trend (p = 0.06) for a reduction in muscularis externa width. There was no difference between groups in the contractile response to acetylcholine, but peak contractility in response to bradykinin (p = 0.02) and angiotensin II (p = 0.03) was significantly greater in the preterm lambs. conclusion: Findings demonstrate that the crypt-villus units are shorter in the ileum of late preterm offspring, but functionally mature with an equivalent cellular composition and normal contractile response to acetylcholine compared with term offspring. The exaggerated contractility to inflammatory mediators evident in the preterm ileum, however, may be of concern.",
keywords = "Development, Gastrointestinal tract, Ileum, Inflammatory bowel disease, Preterm birth, Sheep, Small intestine",
author = "Flores, {Tracey J.} and Nguyen, {Vivian B.} and Widdop, {Robert E.} and Sutherland, {Megan R.} and Polglase, {Graeme R.} and Abud, {Helen E.} and Black, {Mary Jane}",
year = "2018",
month = "1",
day = "29",
doi = "10.3389/fped.2018.00008",
language = "English",
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journal = "Frontiers in Pediatrics",
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}

Morphology and function of the lamb ileum following preterm birth. / Flores, Tracey J.; Nguyen, Vivian B.; Widdop, Robert E.; Sutherland, Megan R.; Polglase, Graeme R.; Abud, Helen E.; Black, Mary Jane.

In: Frontiers in Pediatrics, Vol. 6, 8, 29.01.2018.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Morphology and function of the lamb ileum following preterm birth

AU - Flores, Tracey J.

AU - Nguyen, Vivian B.

AU - Widdop, Robert E.

AU - Sutherland, Megan R.

AU - Polglase, Graeme R.

AU - Abud, Helen E.

AU - Black, Mary Jane

PY - 2018/1/29

Y1 - 2018/1/29

N2 - Background: For infants born moderately/late preterm (32-37 weeks of gestation), immaturity of the intestine has the potential to impact both short- and long-term gastrointestinal function. The aim of this study conducted in sheep was to compare the morphology and smooth muscle contractility of the ileum in term and late preterm lambs. Materials and methods: Lambs delivered preterm (132 days gestation; n = 7) or term (147 days gestation; n = 9) were milk-fed after birth and euthanased at 2 days of age. A segment of distal ileum was collected for analysis of the length and cellular composition of the villi and crypts, smooth muscle width and contractility, and mRNA expression of the cell markers Ki67, lysozyme, mucin 2, synaptophysin, chromogranin A, olfactomedin 4, axis inhibition protein 2, and leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5). results: There was no difference in the proportion of inflammatory, proliferating, apoptotic, enterocyte, or goblet cells between groups, but preterm lambs exhibited a significant upregulation of the stem cell marker LGR5 (p = 0.01). Absolute villus height (term: 1,032 ± 147 μm, preterm: 651 ± 52 μm; p < 0.0001) and crypt depth (term: 153 ± 11 μm, preterm: 133 ± 17 μm; p = 0.01) were significantly shorter in the preterm ileums, with a trend (p = 0.06) for a reduction in muscularis externa width. There was no difference between groups in the contractile response to acetylcholine, but peak contractility in response to bradykinin (p = 0.02) and angiotensin II (p = 0.03) was significantly greater in the preterm lambs. conclusion: Findings demonstrate that the crypt-villus units are shorter in the ileum of late preterm offspring, but functionally mature with an equivalent cellular composition and normal contractile response to acetylcholine compared with term offspring. The exaggerated contractility to inflammatory mediators evident in the preterm ileum, however, may be of concern.

AB - Background: For infants born moderately/late preterm (32-37 weeks of gestation), immaturity of the intestine has the potential to impact both short- and long-term gastrointestinal function. The aim of this study conducted in sheep was to compare the morphology and smooth muscle contractility of the ileum in term and late preterm lambs. Materials and methods: Lambs delivered preterm (132 days gestation; n = 7) or term (147 days gestation; n = 9) were milk-fed after birth and euthanased at 2 days of age. A segment of distal ileum was collected for analysis of the length and cellular composition of the villi and crypts, smooth muscle width and contractility, and mRNA expression of the cell markers Ki67, lysozyme, mucin 2, synaptophysin, chromogranin A, olfactomedin 4, axis inhibition protein 2, and leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5). results: There was no difference in the proportion of inflammatory, proliferating, apoptotic, enterocyte, or goblet cells between groups, but preterm lambs exhibited a significant upregulation of the stem cell marker LGR5 (p = 0.01). Absolute villus height (term: 1,032 ± 147 μm, preterm: 651 ± 52 μm; p < 0.0001) and crypt depth (term: 153 ± 11 μm, preterm: 133 ± 17 μm; p = 0.01) were significantly shorter in the preterm ileums, with a trend (p = 0.06) for a reduction in muscularis externa width. There was no difference between groups in the contractile response to acetylcholine, but peak contractility in response to bradykinin (p = 0.02) and angiotensin II (p = 0.03) was significantly greater in the preterm lambs. conclusion: Findings demonstrate that the crypt-villus units are shorter in the ileum of late preterm offspring, but functionally mature with an equivalent cellular composition and normal contractile response to acetylcholine compared with term offspring. The exaggerated contractility to inflammatory mediators evident in the preterm ileum, however, may be of concern.

KW - Development

KW - Gastrointestinal tract

KW - Ileum

KW - Inflammatory bowel disease

KW - Preterm birth

KW - Sheep

KW - Small intestine

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U2 - 10.3389/fped.2018.00008

DO - 10.3389/fped.2018.00008

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JO - Frontiers in Pediatrics

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