TY - JOUR
T1 - Morphological evaluation of the cerebral blood vessels in the late gestation fetal sheep following hypoxia in utero
AU - Baburamani, Ana Aradhna
AU - Lo, Camden Yeung-Wah
AU - Zakhem, Margie Esmeralda
AU - Walker, David William
PY - 2013
Y1 - 2013
N2 - Hypoxia can significantly contribute to the development of permanent brain injury in the term neonate; however the response of cerebral blood vessels is not well understood. This study aimed to quantitatively measure vascular density and morphology using laminin immunohistochemistry as a marker of blood vessels, and determine the effects of a single, severe bout of hypoxia (umbilical cord occlusion, UCO) late in gestation on the developing cerebrovasculature in fetal sheep. At 124-126days gestation singleton fetal sheep underwent surgery for implantation of catheters and placement of an inflatable cuff around the umbilical cord. A 10min UCO or sham UCO (n=5) occurred at 132days gestation. Fetal brains were collected at 24h (n=5) or 48h (n=4) after UCO for vascular density and morphology analysis of laminin immunohistochemistry. 48h following a single, brief bout of severe hypoxia late in gestation decreased vascular density was seen in the caudate nucleus and no changes in vascular morphology occurred. However closer analysis revealed a significant shift in the frequency of smaller (
AB - Hypoxia can significantly contribute to the development of permanent brain injury in the term neonate; however the response of cerebral blood vessels is not well understood. This study aimed to quantitatively measure vascular density and morphology using laminin immunohistochemistry as a marker of blood vessels, and determine the effects of a single, severe bout of hypoxia (umbilical cord occlusion, UCO) late in gestation on the developing cerebrovasculature in fetal sheep. At 124-126days gestation singleton fetal sheep underwent surgery for implantation of catheters and placement of an inflatable cuff around the umbilical cord. A 10min UCO or sham UCO (n=5) occurred at 132days gestation. Fetal brains were collected at 24h (n=5) or 48h (n=4) after UCO for vascular density and morphology analysis of laminin immunohistochemistry. 48h following a single, brief bout of severe hypoxia late in gestation decreased vascular density was seen in the caudate nucleus and no changes in vascular morphology occurred. However closer analysis revealed a significant shift in the frequency of smaller (
UR - http://www.sciencedirect.com/science/article/pii/S002628621200163X
U2 - 10.1016/j.mvr.2012.09.007
DO - 10.1016/j.mvr.2012.09.007
M3 - Article
SN - 0026-2862
VL - 85
SP - 1
EP - 9
JO - Microvascular Research
JF - Microvascular Research
IS - 1
ER -