Morphologic and mitochondrial characterization of human spermatogenic cells dispersed in wet preparation for testicular sperm extraction: Establishment of a microscopic diagram of developing human spermatogenic cells

Hidenobu Okuda, Akira Tsujimura, Keisuke Yamamoto, Shinichiro Fukuhara, Jiro Nakayama, Hiroshi Kiuchi, Tetsuya Takao, Yasushi Miyagawa, Norio Nonomura, Akihiko Okuyama

Research output: Contribution to journalComment / DebateOtherpeer-review

6 Citations (Scopus)

Abstract

The successful identification of spermatozoa at microdissection testicular sperm extraction is critical and can depend on the judgment of experienced reproductive clinicians. Therefore, we characterized human spermatogenic cells based on stage-specific mitochondrial location and morphologic change by using a vital mitochondrion-specific fluorescent probe, Mitotracker, and stage-specific antibodies to make a useful microscopic diagram.

Original languageEnglish
Pages (from-to)2665-2668
Number of pages4
JournalFertility and Sterility
Volume95
Issue number8
DOIs
Publication statusPublished - 30 Jun 2011
Externally publishedYes

Keywords

  • azoospermia
  • microdissection TESE
  • Mitochondria
  • MitoTracker
  • spermatozoa

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