Morphine-induced Cognitive Dysfunction Model in Zebrafish

Wen-Yang Png; Siew-Ying Mok; Pek-Yee Tang; Satoshi Ogawa; Ishwar Parhar

doi:10.1109/IECBES48179.2021.9398834

Morphine-induced Cognitive Dysfunction Model in Zebrafish

Wen-Yang Png, Siew-Ying Mok, Pek-Yee Tang, Satoshi Ogawa, Ishwar Parhar

Malaysia Jeffrey Cheah Sch of Med & HS

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research › peer-review

1 Citation (Scopus)

Abstract

The neural pathway of opioid use plays a crucial role in drug abuse, cognitive alteration, and abnormal change in neurobehavior. This study aims to investigate the effect of morphine on changes in cognitive aspect through assessment of the startle habituation response in the zebrafish model. Zebrafish embryos were exposed to 0.8 μM morphine for 5 days, starting from 5-hour post-fertilisation (hpf). On 6-day post-fertilisation (dpf), the larvae were transferred to the custom startle apparatus for assessment. The recorded videos were analysed using Toxtrac software. From the analysis, morphine-treated larvae showed impairment in the habituation response, with excessive startle response and non-consistent decrement in habituation. Besides, chaotic movements and preference towards peripheral zone in the larvae treated with morphine also signifying anxiety. We postulate that the morphine regulated pathway are involved in the cognitive alteration and abnormal behaviour of morphine-treated zebrafish. Future studies are required to associate the morphine-induced behaviour to the neural circuitry and firing activities.

Original language	English
Title of host publication	Conference Proceedings: IECBES2020
Subtitle of host publication	Malaysia Virtual Conference
Place of Publication	United States
Publisher	IEEE, Institute of Electrical and Electronics Engineers
Pages	68-71
Number of pages	4
ISBN (Electronic)	9781728142456
DOIs	https://doi.org/10.1109/IECBES48179.2021.9398834
Publication status	Published - 1 Mar 2021
Event	IEEE-EMBS International Conference on Biomedical Engineering and Sciences (IECBES) 2020 - Virtual, Langkawi Island, Malaysia Duration: 1 Mar 2021 → 3 Mar 2021 https://ieeexplore.ieee.org/xpl/conhome/9398728/proceeding (Proceedings)

Conference

Conference	IEEE-EMBS International Conference on Biomedical Engineering and Sciences (IECBES) 2020
Abbreviated title	ICEBES 2020
Country/Territory	Malaysia
City	Virtual, Langkawi Island
Period	1/03/21 → 3/03/21
Internet address	https://ieeexplore.ieee.org/xpl/conhome/9398728/proceeding (Proceedings)

Keywords

Behaviour
Morphine
Schizophrenia

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1109/IECBES48179.2021.9398834

Cite this

@inproceedings{f3d6c94cca8040388dac9124da3e48b2,

title = "Morphine-induced Cognitive Dysfunction Model in Zebrafish",

abstract = "The neural pathway of opioid use plays a crucial role in drug abuse, cognitive alteration, and abnormal change in neurobehavior. This study aims to investigate the effect of morphine on changes in cognitive aspect through assessment of the startle habituation response in the zebrafish model. Zebrafish embryos were exposed to 0.8 μM morphine for 5 days, starting from 5-hour post-fertilisation (hpf). On 6-day post-fertilisation (dpf), the larvae were transferred to the custom startle apparatus for assessment. The recorded videos were analysed using Toxtrac software. From the analysis, morphine-treated larvae showed impairment in the habituation response, with excessive startle response and non-consistent decrement in habituation. Besides, chaotic movements and preference towards peripheral zone in the larvae treated with morphine also signifying anxiety. We postulate that the morphine regulated pathway are involved in the cognitive alteration and abnormal behaviour of morphine-treated zebrafish. Future studies are required to associate the morphine-induced behaviour to the neural circuitry and firing activities.",

keywords = "Behaviour, Morphine, Schizophrenia",

author = "Wen-Yang Png and Siew-Ying Mok and Pek-Yee Tang and Satoshi Ogawa and Ishwar Parhar",

note = "Funding Information: ACKNOWLEDGEMENT We would like to thank Ms. Izzati for maintenance and zebrafish husbandry at BRIMS. This work was funded by UTARRF grants IPSR/RMC/UTARRF/2018-C1/M05 and IPSR/RMC/UTARRF/2019-C1/M01, by MTSF grant 4417/M01, and by FGRS grant FRGS/1/2018/STG03/UTAR/02/1. Publisher Copyright: {\textcopyright} 2021 IEEE. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.; IEEE-EMBS International Conference on Biomedical Engineering and Sciences (IECBES) 2020, ICEBES 2020 ; Conference date: 01-03-2021 Through 03-03-2021",

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doi = "10.1109/IECBES48179.2021.9398834",

language = "English",

pages = "68--71",

booktitle = "Conference Proceedings: IECBES2020",

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Png, W-Y, Mok, S-Y, Tang, P-Y, Ogawa, S & Parhar, I 2021, Morphine-induced Cognitive Dysfunction Model in Zebrafish. in Conference Proceedings: IECBES2020: Malaysia Virtual Conference. IEEE, Institute of Electrical and Electronics Engineers, United States, pp. 68-71, IEEE-EMBS International Conference on Biomedical Engineering and Sciences (IECBES) 2020, Virtual, Langkawi Island, Malaysia, 1/03/21. https://doi.org/10.1109/IECBES48179.2021.9398834

Morphine-induced Cognitive Dysfunction Model in Zebrafish. / Png, Wen-Yang; Mok, Siew-Ying; Tang, Pek-Yee et al.
Conference Proceedings: IECBES2020: Malaysia Virtual Conference. United States: IEEE, Institute of Electrical and Electronics Engineers, 2021. p. 68-71.

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research › peer-review

TY - GEN

T1 - Morphine-induced Cognitive Dysfunction Model in Zebrafish

AU - Png, Wen-Yang

AU - Mok, Siew-Ying

AU - Tang, Pek-Yee

AU - Ogawa, Satoshi

AU - Parhar, Ishwar

N1 - Funding Information: ACKNOWLEDGEMENT We would like to thank Ms. Izzati for maintenance and zebrafish husbandry at BRIMS. This work was funded by UTARRF grants IPSR/RMC/UTARRF/2018-C1/M05 and IPSR/RMC/UTARRF/2019-C1/M01, by MTSF grant 4417/M01, and by FGRS grant FRGS/1/2018/STG03/UTAR/02/1. Publisher Copyright: © 2021 IEEE. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/3/1

Y1 - 2021/3/1

N2 - The neural pathway of opioid use plays a crucial role in drug abuse, cognitive alteration, and abnormal change in neurobehavior. This study aims to investigate the effect of morphine on changes in cognitive aspect through assessment of the startle habituation response in the zebrafish model. Zebrafish embryos were exposed to 0.8 μM morphine for 5 days, starting from 5-hour post-fertilisation (hpf). On 6-day post-fertilisation (dpf), the larvae were transferred to the custom startle apparatus for assessment. The recorded videos were analysed using Toxtrac software. From the analysis, morphine-treated larvae showed impairment in the habituation response, with excessive startle response and non-consistent decrement in habituation. Besides, chaotic movements and preference towards peripheral zone in the larvae treated with morphine also signifying anxiety. We postulate that the morphine regulated pathway are involved in the cognitive alteration and abnormal behaviour of morphine-treated zebrafish. Future studies are required to associate the morphine-induced behaviour to the neural circuitry and firing activities.

AB - The neural pathway of opioid use plays a crucial role in drug abuse, cognitive alteration, and abnormal change in neurobehavior. This study aims to investigate the effect of morphine on changes in cognitive aspect through assessment of the startle habituation response in the zebrafish model. Zebrafish embryos were exposed to 0.8 μM morphine for 5 days, starting from 5-hour post-fertilisation (hpf). On 6-day post-fertilisation (dpf), the larvae were transferred to the custom startle apparatus for assessment. The recorded videos were analysed using Toxtrac software. From the analysis, morphine-treated larvae showed impairment in the habituation response, with excessive startle response and non-consistent decrement in habituation. Besides, chaotic movements and preference towards peripheral zone in the larvae treated with morphine also signifying anxiety. We postulate that the morphine regulated pathway are involved in the cognitive alteration and abnormal behaviour of morphine-treated zebrafish. Future studies are required to associate the morphine-induced behaviour to the neural circuitry and firing activities.

KW - Behaviour

KW - Morphine

KW - Schizophrenia

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U2 - 10.1109/IECBES48179.2021.9398834

DO - 10.1109/IECBES48179.2021.9398834

M3 - Conference Paper

AN - SCOPUS:85104883545

SP - 68

EP - 71

BT - Conference Proceedings: IECBES2020

PB - IEEE, Institute of Electrical and Electronics Engineers

CY - United States

T2 - IEEE-EMBS International Conference on Biomedical Engineering and Sciences (IECBES) 2020

Y2 - 1 March 2021 through 3 March 2021

ER -

Morphine-induced Cognitive Dysfunction Model in Zebrafish

Abstract

Conference

Keywords

UN SDGs

Access to Document

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