Monoclonal antibodies to arginine vasopressin receptor bind to liver, kidney and pituitary membranes

Deborah Trinder, Vincent Mooser, Paddy A. Phillips, A. Ian Smith, David Casley, Colin I. Johnston

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2 Citations (Scopus)

Abstract

1. A vasopressin binding protein purified from rat liver membranes was used to immunize Balb/c mice and, subsequently, for the screening of hybrids raised in two different cell fusions. 2. Three hybrids were obtained which secreted monoclonal antibodies (MoAb) that bound to the purified solubilized receptor as detected by an enzyme‐linked immunosorbent assay technique. All three MoAb immunoprecipitated the purified receptor. 3. In addition, the MoAb bound in a concentration‐dependent manner to crude liver, kidney and anterior pituitary membranes, tissues known to contain arginine vasopressin (AVP) receptors but not to cardiac ventricle membranes which lack AVP receptors. 4. However, the binding of [125I]‐[d(CH2)5, Sar7]AVP (a specific radiolabelled V1 antagonist) to the membrane‐bound receptor was not inhibited by these antibodies. 5. These results suggest that MoAb recognize epitopes which are common to rat liver, kidney and anterior pituitary membranes but are not at the ligand binding site.

Original languageEnglish
Pages (from-to)443-449
Number of pages7
JournalClinical and Experimental Pharmacology and Physiology
Volume20
Issue number6
DOIs
Publication statusPublished - 1 Jan 1993
Externally publishedYes

Keywords

  • arginine vasopressin
  • monoclonal antibodies
  • vasopressin receptor.

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