Monitoring innate immune recruitment by siRNAs in mammalian cells

Michael Paul Marie Gantier; Bryan Raymond George Williams

doi:10.1007/978-1-60761-588-0_2

Monitoring innate immune recruitment by siRNAs in mammalian cells

Michael Paul Marie Gantier, Bryan Raymond George Williams

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other

17 Citations (Scopus)

Abstract

The use of small interfering RNAs (siRNAs) in human therapy may be hindered by the recruitment of nonspecific effects such as the activation of innate immune responses. Recently, several innate immune receptors have been implicated in the detection of siRNAs. This chapter provides a brief overview of the current knowledge of siRNA-induced innate immunity, as well as protocols for the rapid identification of siRNAs with innate immune stimulatory activity.

Original language	English
Title of host publication	Methods in Molecular Biology [P]
Editors	Wei-Ping Min, Thomas Ichim
Place of Publication	USA
Publisher	Humana Press
Pages	21 - 33
Number of pages	13
Volume	623
Edition	1st
DOIs	https://doi.org/10.1007/978-1-60761-588-0_2
Publication status	Published - 2010

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10.1007/978-1-60761-588-0_2

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title = "Monitoring innate immune recruitment by siRNAs in mammalian cells",

abstract = "The use of small interfering RNAs (siRNAs) in human therapy may be hindered by the recruitment of nonspecific effects such as the activation of innate immune responses. Recently, several innate immune receptors have been implicated in the detection of siRNAs. This chapter provides a brief overview of the current knowledge of siRNA-induced innate immunity, as well as protocols for the rapid identification of siRNAs with innate immune stimulatory activity.",

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AB - The use of small interfering RNAs (siRNAs) in human therapy may be hindered by the recruitment of nonspecific effects such as the activation of innate immune responses. Recently, several innate immune receptors have been implicated in the detection of siRNAs. This chapter provides a brief overview of the current knowledge of siRNA-induced innate immunity, as well as protocols for the rapid identification of siRNAs with innate immune stimulatory activity.

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BT - Methods in Molecular Biology [P]

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PB - Humana Press

CY - USA

ER -

Monitoring innate immune recruitment by siRNAs in mammalian cells

Abstract

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