The use of small interfering RNAs (siRNAs) in human therapy may be hindered by the recruitment of nonspecific effects such as the activation of innate immune responses. Recently, several innate immune receptors have been implicated in the detection of siRNAs. This chapter provides a brief overview of the current knowledge of siRNA-induced innate immunity, as well as protocols for the rapid identification of siRNAs with innate immune stimulatory activity.
|Title of host publication||Methods in Molecular Biology [P]|
|Editors||Wei-Ping Min, Thomas Ichim|
|Place of Publication||USA|
|Pages||21 - 33|
|Number of pages||13|
|Publication status||Published - 2010|
Gantier, M. P. M., & Williams, B. R. G. (2010). Monitoring innate immune recruitment by siRNAs in mammalian cells. In W-P. Min, & T. Ichim (Eds.), Methods in Molecular Biology [P] (First ed., Vol. 623, pp. 21 - 33). Humana Press. https://doi.org/10.1007/978-1-60761-588-0_2