Monitoring innate immune recruitment by siRNAs in mammalian cells

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Abstract

The use of small interfering RNAs (siRNAs) in human therapy may be hindered by the recruitment of nonspecific effects such as the activation of innate immune responses. Recently, several innate immune receptors have been implicated in the detection of siRNAs. This chapter provides a brief overview of the current knowledge of siRNA-induced innate immunity, as well as protocols for the rapid identification of siRNAs with innate immune stimulatory activity.
Original languageEnglish
Title of host publicationMethods in Molecular Biology [P]
EditorsWei-Ping Min, Thomas Ichim
Place of PublicationUSA
PublisherHumana Press
Pages21 - 33
Number of pages13
Volume623
EditionFirst
ISBN (Print)1064-3745
DOIs
Publication statusPublished - 2010

Cite this

Gantier, M. P. M., & Williams, B. R. G. (2010). Monitoring innate immune recruitment by siRNAs in mammalian cells. In W-P. Min, & T. Ichim (Eds.), Methods in Molecular Biology [P] (First ed., Vol. 623, pp. 21 - 33). Humana Press. https://doi.org/10.1007/978-1-60761-588-0_2