Monitoring dendritic cell activation and maturation

Christophe Macri, Ben Fancke, Kristen J. Radford, Meredith O’Keeffe

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

Since the 1997 discovery that the first identified human homolog of Drosophila Toll could activate the innate immune system, the innate arm of immunity has rapidly taken on a new light as an important player in the recognition of pathogens and damaged self. The recognition of danger by dendritic cells (DC) is a crucial step in activating the adaptive immune system. Different DC express varied subsets of pattern recognition receptors (PRR), enabling both overlap and exclusivity in the recognition of danger signals by DC. PRR-mediated DC maturation and activation can be measured by changes in the surface expression of costimulatory as well as coinhibitory molecules, changes in size and shape of the DC and by their production of different cytokines.

Original languageEnglish
Title of host publicationAntigen Processing
Subtitle of host publicationMethods and Protocols
EditorsPeter van Endert
Place of PublicationNew York, NY
PublisherHumana Press
Chapter28
Pages403-418
Number of pages16
Edition2nd
ISBN (Electronic)9781493994502
ISBN (Print)9781493994496
DOIs
Publication statusPublished - 31 May 2019

Publication series

NameMethods in Molecular Biology
Volume1988
ISSN (Print)1064-3745

Keywords

  • Dendritic cell
  • Dendritic cell activation
  • Dendritic cell maturation
  • Innate immune responses
  • Pattern recognition receptor

Cite this

Macri, C., Fancke, B., Radford, K. J., & O’Keeffe, M. (2019). Monitoring dendritic cell activation and maturation. In P. van Endert (Ed.), Antigen Processing: Methods and Protocols (2nd ed., pp. 403-418). (Methods in Molecular Biology; Vol. 1988). Humana Press. https://doi.org/10.1007/978-1-4939-9450-2_28