Monitoring dendritic cell activation and maturation

Christophe Macri; Ben Fancke; Kristen J. Radford; Meredith O’Keeffe

doi:10.1007/978-1-4939-9450-2_28

Monitoring dendritic cell activation and maturation

Christophe Macri, Ben Fancke, Kristen J. Radford, Meredith O’Keeffe

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

11 Citations (Scopus)

Abstract

Since the 1997 discovery that the first identified human homolog of Drosophila Toll could activate the innate immune system, the innate arm of immunity has rapidly taken on a new light as an important player in the recognition of pathogens and damaged self. The recognition of danger by dendritic cells (DC) is a crucial step in activating the adaptive immune system. Different DC express varied subsets of pattern recognition receptors (PRR), enabling both overlap and exclusivity in the recognition of danger signals by DC. PRR-mediated DC maturation and activation can be measured by changes in the surface expression of costimulatory as well as coinhibitory molecules, changes in size and shape of the DC and by their production of different cytokines.

Original language	English
Title of host publication	Antigen Processing
Subtitle of host publication	Methods and Protocols
Editors	Peter van Endert
Place of Publication	New York, NY
Publisher	Humana Press
Chapter	28
Pages	403-418
Number of pages	16
Edition	2nd
ISBN (Electronic)	9781493994502
ISBN (Print)	9781493994496
DOIs	https://doi.org/10.1007/978-1-4939-9450-2_28
Publication status	Published - 31 May 2019

Publication series

Name	Methods in Molecular Biology
Volume	1988
ISSN (Print)	1064-3745

Keywords

Dendritic cell
Dendritic cell activation
Dendritic cell maturation
Innate immune responses
Pattern recognition receptor

Access to Document

10.1007/978-1-4939-9450-2_28

Cite this

@inbook{045532a827714e26aaf70cfa076e1675,

title = "Monitoring dendritic cell activation and maturation",

abstract = "Since the 1997 discovery that the first identified human homolog of Drosophila Toll could activate the innate immune system, the innate arm of immunity has rapidly taken on a new light as an important player in the recognition of pathogens and damaged self. The recognition of danger by dendritic cells (DC) is a crucial step in activating the adaptive immune system. Different DC express varied subsets of pattern recognition receptors (PRR), enabling both overlap and exclusivity in the recognition of danger signals by DC. PRR-mediated DC maturation and activation can be measured by changes in the surface expression of costimulatory as well as coinhibitory molecules, changes in size and shape of the DC and by their production of different cytokines.",

keywords = "Dendritic cell, Dendritic cell activation, Dendritic cell maturation, Innate immune responses, Pattern recognition receptor",

author = "Christophe Macri and Ben Fancke and Radford, {Kristen J.} and Meredith O{\textquoteright}Keeffe",

year = "2019",

month = may,

day = "31",

doi = "10.1007/978-1-4939-9450-2_28",

language = "English",

isbn = "9781493994496",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

pages = "403--418",

editor = "{van Endert}, Peter",

booktitle = "Antigen Processing",

address = "United States of America",

edition = "2nd",

}

Monitoring dendritic cell activation and maturation. / Macri, Christophe; Fancke, Ben; Radford, Kristen J. et al.
Antigen Processing: Methods and Protocols. ed. / Peter van Endert. 2nd. ed. New York, NY: Humana Press, 2019. p. 403-418 (Methods in Molecular Biology; Vol. 1988).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

TY - CHAP

T1 - Monitoring dendritic cell activation and maturation

AU - Macri, Christophe

AU - Fancke, Ben

AU - Radford, Kristen J.

AU - O’Keeffe, Meredith

PY - 2019/5/31

Y1 - 2019/5/31

N2 - Since the 1997 discovery that the first identified human homolog of Drosophila Toll could activate the innate immune system, the innate arm of immunity has rapidly taken on a new light as an important player in the recognition of pathogens and damaged self. The recognition of danger by dendritic cells (DC) is a crucial step in activating the adaptive immune system. Different DC express varied subsets of pattern recognition receptors (PRR), enabling both overlap and exclusivity in the recognition of danger signals by DC. PRR-mediated DC maturation and activation can be measured by changes in the surface expression of costimulatory as well as coinhibitory molecules, changes in size and shape of the DC and by their production of different cytokines.

AB - Since the 1997 discovery that the first identified human homolog of Drosophila Toll could activate the innate immune system, the innate arm of immunity has rapidly taken on a new light as an important player in the recognition of pathogens and damaged self. The recognition of danger by dendritic cells (DC) is a crucial step in activating the adaptive immune system. Different DC express varied subsets of pattern recognition receptors (PRR), enabling both overlap and exclusivity in the recognition of danger signals by DC. PRR-mediated DC maturation and activation can be measured by changes in the surface expression of costimulatory as well as coinhibitory molecules, changes in size and shape of the DC and by their production of different cytokines.

KW - Dendritic cell

KW - Dendritic cell activation

KW - Dendritic cell maturation

KW - Innate immune responses

KW - Pattern recognition receptor

UR - http://www.scopus.com/inward/record.url?scp=85066791191&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-9450-2_28

DO - 10.1007/978-1-4939-9450-2_28

M3 - Chapter (Book)

AN - SCOPUS:85066791191

SN - 9781493994496

T3 - Methods in Molecular Biology

SP - 403

EP - 418

BT - Antigen Processing

A2 - van Endert, Peter

PB - Humana Press

CY - New York, NY

ER -

Monitoring dendritic cell activation and maturation

Abstract

Publication series

Keywords

Access to Document

Other files and links

Research output

Monitoring dendritic cell activation and maturation

Cite this