Monitoring dendritic cell activation and maturation

Ben Fancke, Meredith O'Keeffe

    Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

    4 Citations (Scopus)

    Abstract

    Since the 1997 discovery that the fi rst identified human homologue of Drosophila Toll could activate the innate immune system, the innate arm of immunity has rapidly taken on a new light as an important player in the recognition of pathogens and damaged self. The recognition of danger by dendritic cells (DC) is a crucial step in activating the adaptive immune system. Different DC express varied subsets of pattern recognition receptors (PRR), enabling both overlap and exclusivity in the recognition of danger signals by DC. PRR-mediated DC maturation and activation can be measured by changes in the surface expression of costimulatory molecules and changes in size and shape of the DC and by their production of different cytokines.

    Original languageEnglish
    Title of host publicationAntigen Processing: Methods and Protocols
    EditorsPeter van Endert
    Place of PublicationNew York NY USA
    PublisherHumana Press
    Chapter26
    Pages359-370
    Number of pages12
    Edition1
    ISBN (Electronic)9781627032186
    ISBN (Print)9781627032179
    DOIs
    Publication statusPublished - 2013

    Publication series

    NameMethods in Molecular Biology
    PublisherHuman Press
    Volume960
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029

    Keywords

    • Dendritic cell
    • Dendritic cell activation
    • Dendritic cell maturation
    • Innate immune responses
    • Pattern recognition receptor
    • Monitoring dendritic cell activation and maturation

      Macri, C., Fancke, B., Radford, K. J. & O’Keeffe, M., 31 May 2019, Antigen Processing: Methods and Protocols. van Endert, P. (ed.). 2nd ed. New York, NY: Humana Press, p. 403-418 16 p. (Methods in Molecular Biology; vol. 1988).

      Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

      11 Citations (Scopus)

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