Monashia flava gen. Nov., sp. nov., An actinobacterium of the family Intrasporangiaceae

Adzzie Shazleen Azman, Nurullhudda Zainal, Nurul Syakima Ab Mutalib, Wai Fong Yin, Kok Gan Chan, Learn Han Lee

    Research output: Contribution to journalArticleResearchpeer-review

    6 Citations (Scopus)

    Abstract

    A novel actinobacterial strain, MUSC 78T, was isolated from a mangrove soil collected from Peninsular Malaysia. The taxonomic status of this strain was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain MUSC 78T represented a novel lineage within the class Actinobacteria. Strain MUSC 78T formed a distinct clade in the family Intrasporangiaceae and was related most closely to members of the genera Terrabacter (98.3–96.8 % 16S rRNA gene sequence similarity), Intrasporangium (98.2– 96.8 %), Humibacillus (97.2 %), Janibacter (97.0–95.3 %), Terracoccus (96.8 %), Kribbia (96.6 %), Phycicoccus (96.2–94.7 %), Knoellia (96.1–94.8 %), Tetrasphaera (96.0–94.9 %) and Lapillicoccus (95.9 %). Cells were irregular rod-shaped or cocci and stained Grampositive. The cell-wall peptidoglycan type was A3γ, with LL-diaminopimelic acid as the diagnostic diamino acid. The main cell-wall sugar was mannose and lower amounts of galactose and rhamnose were present. The predominant menaquinone was MK-8(H4). The polar lipid profile consisted of phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipid. The predominant fatty acids were iso-C15: 0, anteiso-C15: 0 and iso-C16: 0.The DNA G+C content was 73.1 mol%. Based on this polyphasic study, MUSC 78T exhibited phylogenetic and phenotypic differences from members of the genera of the family Intrasporangiaceae, and therefore a novel species of a new genus, Monashia flava gen. nov., sp. nov., is proposed. The type strain of Monashia flava is MUSC 78T (=DSM 29621T=MCCC 1K00454T=NBRC 110749T).

    Original languageEnglish
    Pages (from-to)554-561
    Number of pages8
    JournalInternational Journal of Systematic and Evolutionary Microbiology
    Volume66
    Issue number2
    DOIs
    Publication statusPublished - 1 Feb 2016

    Cite this