Molecular mechanisms responsible for microglia-derived protection of Sprague-Dawley rat brain cells during in vitro ischemia

Yen Zhen Lu, Chi Hsin Lin, Fu Chou Cheng, Chi Mei Hsueh

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Abstract

Microglia-derived protection of brain cells (microglia, astrocytes, and neurons) during in vitro ischemic stress (deprivation of glucose, oxygen, and serum) was determined. Trypan blue exclusion assay, immunoblocking assay, Western blot analysis, and ELISA assay were used to determine the molecular mechanisms responsible for the microglia-derived protection. Results demonstrated that supernatants from the ischemic microglia protected all three cell-types from ischemia-induced damage by releasing the transforming growth factor-β1 (TGF-β1) and glial cell line-derived neurotrophic factor (GDNF). The protection of microglia was TGF-β1 related, whereas astrocytes protection was GDNF-dependent. The protection of neurons was TGF-β1 and GDNF independent, and the molecular nature responsible for their protection remains to be determined. These results indicate contribution from the surrounding cells and the types of receptors expressed on different brain cells probably also play an important role in determining their fate against ischemia.

Original languageEnglish
Pages (from-to)159-164
Number of pages6
JournalNeuroscience Letters
Volume373
Issue number2
DOIs
Publication statusPublished - 10 Jan 2005
Externally publishedYes

Keywords

  • Astrocyte
  • GDNF
  • In vitro ischemia
  • Microglia
  • Neuron
  • TGB-β1

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