Molecular imaging of activated platelets allows the detection of pulmonary embolism with magnetic resonance imaging

Timo Heidt, Simon Ehrismann, Jan Bernd Hovener, Irene Neudorfer, Ingo Hilgendorf, Marco Reisert, Christoph Eugen Hagemeyer, Andreas Zirlik, Jochen O Reinohl, Christoph Bode, Karlheinz Peter, Dominik von Elverfeldt, Constantin Von Zur Muhlen

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Early and reliable detection of pulmonary embolism (PE) is critical for improving patient morbidity and mortality. The desire for low-threshold screening for pulmonary embolism is contradicted by unfavorable radiation of currently used computed tomography or nuclear techniques, while standard magnetic resonance imaging still struggles to provide sufficient diagnostic sensitivity in the lung. In this study we evaluate a molecular-targeted contrast agent against activated platelets for non-invasive detection of murine pulmonary thromboembolism using magnetic resonance imaging. By intravenous injection of human thrombin, pulmonary thromboembolism were consistently induced as confirmed by immunohistochemistry of the lung. Magnetic resonance imaging after thrombin injection showed local tissue edema in T2* weighted images which co-localized with the histological presence of pulmonary thromboembolism. Furthermore, injection of a functionalized contrast agent targeting activated platelets provided sensitive evidence of focal accumulation of activated platelets within the edematous area, which, ex vivo, correlated well with the size of the pulmonary embolism. In summary, we here show delivery and specific binding of a functionalized molecular contrast agent against activated platelets for targeting pulmonary thromboembolism. Going forward, molecular imaging may provide new opportunities to increase sensitivity of magnetic resonance imaging for detection of pulmonary embolism.
Original languageEnglish
Article number25044
Number of pages9
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016

Cite this

Heidt, T., Ehrismann, S., Hovener, J. B., Neudorfer, I., Hilgendorf, I., Reisert, M., ... Von Zur Muhlen, C. (2016). Molecular imaging of activated platelets allows the detection of pulmonary embolism with magnetic resonance imaging. Scientific Reports, 6, [25044]. https://doi.org/10.1038/srep25044
Heidt, Timo ; Ehrismann, Simon ; Hovener, Jan Bernd ; Neudorfer, Irene ; Hilgendorf, Ingo ; Reisert, Marco ; Hagemeyer, Christoph Eugen ; Zirlik, Andreas ; Reinohl, Jochen O ; Bode, Christoph ; Peter, Karlheinz ; von Elverfeldt, Dominik ; Von Zur Muhlen, Constantin. / Molecular imaging of activated platelets allows the detection of pulmonary embolism with magnetic resonance imaging. In: Scientific Reports. 2016 ; Vol. 6.
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title = "Molecular imaging of activated platelets allows the detection of pulmonary embolism with magnetic resonance imaging",
abstract = "Early and reliable detection of pulmonary embolism (PE) is critical for improving patient morbidity and mortality. The desire for low-threshold screening for pulmonary embolism is contradicted by unfavorable radiation of currently used computed tomography or nuclear techniques, while standard magnetic resonance imaging still struggles to provide sufficient diagnostic sensitivity in the lung. In this study we evaluate a molecular-targeted contrast agent against activated platelets for non-invasive detection of murine pulmonary thromboembolism using magnetic resonance imaging. By intravenous injection of human thrombin, pulmonary thromboembolism were consistently induced as confirmed by immunohistochemistry of the lung. Magnetic resonance imaging after thrombin injection showed local tissue edema in T2* weighted images which co-localized with the histological presence of pulmonary thromboembolism. Furthermore, injection of a functionalized contrast agent targeting activated platelets provided sensitive evidence of focal accumulation of activated platelets within the edematous area, which, ex vivo, correlated well with the size of the pulmonary embolism. In summary, we here show delivery and specific binding of a functionalized molecular contrast agent against activated platelets for targeting pulmonary thromboembolism. Going forward, molecular imaging may provide new opportunities to increase sensitivity of magnetic resonance imaging for detection of pulmonary embolism.",
author = "Timo Heidt and Simon Ehrismann and Hovener, {Jan Bernd} and Irene Neudorfer and Ingo Hilgendorf and Marco Reisert and Hagemeyer, {Christoph Eugen} and Andreas Zirlik and Reinohl, {Jochen O} and Christoph Bode and Karlheinz Peter and {von Elverfeldt}, Dominik and {Von Zur Muhlen}, Constantin",
year = "2016",
doi = "10.1038/srep25044",
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Heidt, T, Ehrismann, S, Hovener, JB, Neudorfer, I, Hilgendorf, I, Reisert, M, Hagemeyer, CE, Zirlik, A, Reinohl, JO, Bode, C, Peter, K, von Elverfeldt, D & Von Zur Muhlen, C 2016, 'Molecular imaging of activated platelets allows the detection of pulmonary embolism with magnetic resonance imaging' Scientific Reports, vol. 6, 25044. https://doi.org/10.1038/srep25044

Molecular imaging of activated platelets allows the detection of pulmonary embolism with magnetic resonance imaging. / Heidt, Timo; Ehrismann, Simon; Hovener, Jan Bernd; Neudorfer, Irene; Hilgendorf, Ingo; Reisert, Marco; Hagemeyer, Christoph Eugen; Zirlik, Andreas; Reinohl, Jochen O; Bode, Christoph; Peter, Karlheinz; von Elverfeldt, Dominik; Von Zur Muhlen, Constantin.

In: Scientific Reports, Vol. 6, 25044, 2016.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Molecular imaging of activated platelets allows the detection of pulmonary embolism with magnetic resonance imaging

AU - Heidt, Timo

AU - Ehrismann, Simon

AU - Hovener, Jan Bernd

AU - Neudorfer, Irene

AU - Hilgendorf, Ingo

AU - Reisert, Marco

AU - Hagemeyer, Christoph Eugen

AU - Zirlik, Andreas

AU - Reinohl, Jochen O

AU - Bode, Christoph

AU - Peter, Karlheinz

AU - von Elverfeldt, Dominik

AU - Von Zur Muhlen, Constantin

PY - 2016

Y1 - 2016

N2 - Early and reliable detection of pulmonary embolism (PE) is critical for improving patient morbidity and mortality. The desire for low-threshold screening for pulmonary embolism is contradicted by unfavorable radiation of currently used computed tomography or nuclear techniques, while standard magnetic resonance imaging still struggles to provide sufficient diagnostic sensitivity in the lung. In this study we evaluate a molecular-targeted contrast agent against activated platelets for non-invasive detection of murine pulmonary thromboembolism using magnetic resonance imaging. By intravenous injection of human thrombin, pulmonary thromboembolism were consistently induced as confirmed by immunohistochemistry of the lung. Magnetic resonance imaging after thrombin injection showed local tissue edema in T2* weighted images which co-localized with the histological presence of pulmonary thromboembolism. Furthermore, injection of a functionalized contrast agent targeting activated platelets provided sensitive evidence of focal accumulation of activated platelets within the edematous area, which, ex vivo, correlated well with the size of the pulmonary embolism. In summary, we here show delivery and specific binding of a functionalized molecular contrast agent against activated platelets for targeting pulmonary thromboembolism. Going forward, molecular imaging may provide new opportunities to increase sensitivity of magnetic resonance imaging for detection of pulmonary embolism.

AB - Early and reliable detection of pulmonary embolism (PE) is critical for improving patient morbidity and mortality. The desire for low-threshold screening for pulmonary embolism is contradicted by unfavorable radiation of currently used computed tomography or nuclear techniques, while standard magnetic resonance imaging still struggles to provide sufficient diagnostic sensitivity in the lung. In this study we evaluate a molecular-targeted contrast agent against activated platelets for non-invasive detection of murine pulmonary thromboembolism using magnetic resonance imaging. By intravenous injection of human thrombin, pulmonary thromboembolism were consistently induced as confirmed by immunohistochemistry of the lung. Magnetic resonance imaging after thrombin injection showed local tissue edema in T2* weighted images which co-localized with the histological presence of pulmonary thromboembolism. Furthermore, injection of a functionalized contrast agent targeting activated platelets provided sensitive evidence of focal accumulation of activated platelets within the edematous area, which, ex vivo, correlated well with the size of the pulmonary embolism. In summary, we here show delivery and specific binding of a functionalized molecular contrast agent against activated platelets for targeting pulmonary thromboembolism. Going forward, molecular imaging may provide new opportunities to increase sensitivity of magnetic resonance imaging for detection of pulmonary embolism.

UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853725/

UR - https://www.ncbi.nlm.nih.gov/pubmed/27138487

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DO - 10.1038/srep25044

M3 - Article

VL - 6

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 25044

ER -