Abstract
| Original language | English |
|---|---|
| Article number | 25044 |
| Number of pages | 9 |
| Journal | Scientific Reports |
| Volume | 6 |
| DOIs | |
| Publication status | Published - 2016 |
Cite this
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Molecular imaging of activated platelets allows the detection of pulmonary embolism with magnetic resonance imaging. / Heidt, Timo; Ehrismann, Simon; Hovener, Jan Bernd; Neudorfer, Irene; Hilgendorf, Ingo; Reisert, Marco; Hagemeyer, Christoph Eugen; Zirlik, Andreas; Reinohl, Jochen O; Bode, Christoph; Peter, Karlheinz; von Elverfeldt, Dominik; Von Zur Muhlen, Constantin.
In: Scientific Reports, Vol. 6, 25044, 2016.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Molecular imaging of activated platelets allows the detection of pulmonary embolism with magnetic resonance imaging
AU - Heidt, Timo
AU - Ehrismann, Simon
AU - Hovener, Jan Bernd
AU - Neudorfer, Irene
AU - Hilgendorf, Ingo
AU - Reisert, Marco
AU - Hagemeyer, Christoph Eugen
AU - Zirlik, Andreas
AU - Reinohl, Jochen O
AU - Bode, Christoph
AU - Peter, Karlheinz
AU - von Elverfeldt, Dominik
AU - Von Zur Muhlen, Constantin
PY - 2016
Y1 - 2016
N2 - Early and reliable detection of pulmonary embolism (PE) is critical for improving patient morbidity and mortality. The desire for low-threshold screening for pulmonary embolism is contradicted by unfavorable radiation of currently used computed tomography or nuclear techniques, while standard magnetic resonance imaging still struggles to provide sufficient diagnostic sensitivity in the lung. In this study we evaluate a molecular-targeted contrast agent against activated platelets for non-invasive detection of murine pulmonary thromboembolism using magnetic resonance imaging. By intravenous injection of human thrombin, pulmonary thromboembolism were consistently induced as confirmed by immunohistochemistry of the lung. Magnetic resonance imaging after thrombin injection showed local tissue edema in T2* weighted images which co-localized with the histological presence of pulmonary thromboembolism. Furthermore, injection of a functionalized contrast agent targeting activated platelets provided sensitive evidence of focal accumulation of activated platelets within the edematous area, which, ex vivo, correlated well with the size of the pulmonary embolism. In summary, we here show delivery and specific binding of a functionalized molecular contrast agent against activated platelets for targeting pulmonary thromboembolism. Going forward, molecular imaging may provide new opportunities to increase sensitivity of magnetic resonance imaging for detection of pulmonary embolism.
AB - Early and reliable detection of pulmonary embolism (PE) is critical for improving patient morbidity and mortality. The desire for low-threshold screening for pulmonary embolism is contradicted by unfavorable radiation of currently used computed tomography or nuclear techniques, while standard magnetic resonance imaging still struggles to provide sufficient diagnostic sensitivity in the lung. In this study we evaluate a molecular-targeted contrast agent against activated platelets for non-invasive detection of murine pulmonary thromboembolism using magnetic resonance imaging. By intravenous injection of human thrombin, pulmonary thromboembolism were consistently induced as confirmed by immunohistochemistry of the lung. Magnetic resonance imaging after thrombin injection showed local tissue edema in T2* weighted images which co-localized with the histological presence of pulmonary thromboembolism. Furthermore, injection of a functionalized contrast agent targeting activated platelets provided sensitive evidence of focal accumulation of activated platelets within the edematous area, which, ex vivo, correlated well with the size of the pulmonary embolism. In summary, we here show delivery and specific binding of a functionalized molecular contrast agent against activated platelets for targeting pulmonary thromboembolism. Going forward, molecular imaging may provide new opportunities to increase sensitivity of magnetic resonance imaging for detection of pulmonary embolism.
UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853725/
UR - https://www.ncbi.nlm.nih.gov/pubmed/27138487
U2 - 10.1038/srep25044
DO - 10.1038/srep25044
M3 - Article
VL - 6
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 25044
ER -