Molecular diagnosis of bacterial vaginosis: Does adjustment for total bacterial load or human cellular content improve diagnostic performance?

E. L. Plummer, S. M. Garland, C. S. Bradshaw, M. G. Law, L. A. Vodstrcil, J. S. Hocking, C. K. Fairley, S. N. Tabrizi

Research output: Contribution to journalArticleResearchpeer-review

Abstract

We investigated the utility of quantitative PCR assays for diagnosis of bacterial vaginosis and found that while the best model utilized bacterial copy number adjusted for total bacterial load (sensitivity = 98%, specificity = 93%, AUC = 0.95[95%CI = 0.93,0.97]), adjusting for total bacterial or human cell load did not consistently increase the diagnostic performance of the assays.

Original languageEnglish
Pages (from-to)66-68
Number of pages3
JournalJournal of Microbiological Methods
Volume133
DOIs
Publication statusPublished - 1 Feb 2017

Keywords

  • Bacterial vaginosis
  • Molecular diagnosis
  • qPCR

Cite this

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title = "Molecular diagnosis of bacterial vaginosis: Does adjustment for total bacterial load or human cellular content improve diagnostic performance?",
abstract = "We investigated the utility of quantitative PCR assays for diagnosis of bacterial vaginosis and found that while the best model utilized bacterial copy number adjusted for total bacterial load (sensitivity = 98{\%}, specificity = 93{\%}, AUC = 0.95[95{\%}CI = 0.93,0.97]), adjusting for total bacterial or human cell load did not consistently increase the diagnostic performance of the assays.",
keywords = "Bacterial vaginosis, Molecular diagnosis, qPCR",
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year = "2017",
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language = "English",
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journal = "Journal of Microbiological Methods",
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Molecular diagnosis of bacterial vaginosis : Does adjustment for total bacterial load or human cellular content improve diagnostic performance? / Plummer, E. L.; Garland, S. M.; Bradshaw, C. S.; Law, M. G.; Vodstrcil, L. A.; Hocking, J. S.; Fairley, C. K.; Tabrizi, S. N.

In: Journal of Microbiological Methods, Vol. 133, 01.02.2017, p. 66-68.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Molecular diagnosis of bacterial vaginosis

T2 - Does adjustment for total bacterial load or human cellular content improve diagnostic performance?

AU - Plummer, E. L.

AU - Garland, S. M.

AU - Bradshaw, C. S.

AU - Law, M. G.

AU - Vodstrcil, L. A.

AU - Hocking, J. S.

AU - Fairley, C. K.

AU - Tabrizi, S. N.

PY - 2017/2/1

Y1 - 2017/2/1

N2 - We investigated the utility of quantitative PCR assays for diagnosis of bacterial vaginosis and found that while the best model utilized bacterial copy number adjusted for total bacterial load (sensitivity = 98%, specificity = 93%, AUC = 0.95[95%CI = 0.93,0.97]), adjusting for total bacterial or human cell load did not consistently increase the diagnostic performance of the assays.

AB - We investigated the utility of quantitative PCR assays for diagnosis of bacterial vaginosis and found that while the best model utilized bacterial copy number adjusted for total bacterial load (sensitivity = 98%, specificity = 93%, AUC = 0.95[95%CI = 0.93,0.97]), adjusting for total bacterial or human cell load did not consistently increase the diagnostic performance of the assays.

KW - Bacterial vaginosis

KW - Molecular diagnosis

KW - qPCR

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U2 - 10.1016/j.mimet.2016.12.024

DO - 10.1016/j.mimet.2016.12.024

M3 - Article

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