TY - JOUR
T1 - Molecular detection of class 1 integron-associated gene cassettes in KPC-2-producing klebsiella pneumoniae clones by whole-genome sequencing
AU - Fuga, Bruna
AU - Royer, Sabrina
AU - Campos, Paola Amaral De
AU - Ferreira, Melina Lorraine
AU - Rossi, Iara
AU - Machado, Luiz Gustavo
AU - Cerdeira, Louise Teixeira
AU - Fonseca Batistão, Deivid William Da
AU - Brito, Cristiane Silveira De
AU - Lincopan, Nilton
AU - Gontijo-Filho, Paulo Pinto
AU - Ribas, Rosineide Marques
PY - 2019/10
Y1 - 2019/10
N2 - The dissemination of antimicrobial resistance genes and the bacterium that harbor them have increasingly become a public concern, especially in low- and middle-income countries. The present study used whole-genome sequencing to analyze 10 KPC-2-producing Klebsiella pneumoniae isolates obtained from clinical specimens originated from Brazilian hospitals. The study documents a relevant "snapshot" of the presence of class 1 integrons in 90% of the strains presenting different gene cassettes (dfrA30, dfrA15, dfrA12, dfrA14, aadA1, aadA2, and aac(6′)Iq), associated or not with transposons. Two strains presented nonclassical integron (lacking the normal 3′conserved segment). In general, most strains showed a complex resistome, characterizing them as highly resistant. Integrons, a genetically stable and efficient system, confer to bacteria as highly adaptive and low cost evolution potential to bacteria, even more serious when associated with high-risk clones, indicating an urgent need for control and prevention strategies to avoid the spread of resistance determinants in Brazil. Despite this, although the class 1 integron identified in the KPC-2-producing K. pneumoniae clones is important, our findings suggest that other elements probably have a greater impact on the spread of antimicrobial resistance, since many of these important genes were not related to this cassette.
AB - The dissemination of antimicrobial resistance genes and the bacterium that harbor them have increasingly become a public concern, especially in low- and middle-income countries. The present study used whole-genome sequencing to analyze 10 KPC-2-producing Klebsiella pneumoniae isolates obtained from clinical specimens originated from Brazilian hospitals. The study documents a relevant "snapshot" of the presence of class 1 integrons in 90% of the strains presenting different gene cassettes (dfrA30, dfrA15, dfrA12, dfrA14, aadA1, aadA2, and aac(6′)Iq), associated or not with transposons. Two strains presented nonclassical integron (lacking the normal 3′conserved segment). In general, most strains showed a complex resistome, characterizing them as highly resistant. Integrons, a genetically stable and efficient system, confer to bacteria as highly adaptive and low cost evolution potential to bacteria, even more serious when associated with high-risk clones, indicating an urgent need for control and prevention strategies to avoid the spread of resistance determinants in Brazil. Despite this, although the class 1 integron identified in the KPC-2-producing K. pneumoniae clones is important, our findings suggest that other elements probably have a greater impact on the spread of antimicrobial resistance, since many of these important genes were not related to this cassette.
KW - class 1 integron
KW - Klebsiella pneumoniae
KW - microbial drug resistance
UR - http://www.scopus.com/inward/record.url?scp=85073183854&partnerID=8YFLogxK
U2 - 10.1089/mdr.2018.0437
DO - 10.1089/mdr.2018.0437
M3 - Article
C2 - 31074706
AN - SCOPUS:85073183854
SN - 1076-6294
VL - 25
SP - 1127
EP - 1131
JO - Microbial Drug Resistance
JF - Microbial Drug Resistance
IS - 8
ER -