TY - JOUR
T1 - Molecular deletion of 9p sequences in non‐small cell lung cancer and malignant mesothelioma
AU - Center, Rob
AU - Lukeis, Robyn
AU - Dietzsch, Erin
AU - Gillespie, Matthew
AU - Garson, O. Margaret
PY - 1993/1/1
Y1 - 1993/1/1
N2 - Previously we have reported non‐random cytogenetic abnormalities involving the short arm of chromosome 9 (9p) in the majority of primary non‐small cell lung cancer (NSCLC) patient samples, which indicated loss of DNA sequences. In another lung tumor, pleural malignant mesothelioma (MM), cytogenetic changes also include apparent deletions of 9p. To define the location and extent of deletions of 9p in NSCLC and MM, Southern blot analyses on six NSCLC and five MM cell lines using molecular probes to 9p loci (IFNA, IFNBI, D9S3, and D9S19) were performed, and DNA dosage was determined by densitometry. Our data demonstrated reduced dosage of 9p sequences in three of six NSCLC and four of five MM lines. A homozygous deletion of D9S3 was found in one NSCLC and one MM cell line. The region of common loss overlapped the D9S3 locus and was flanked by the IFNBI and D9S19 loci. IFNBI has previously been localized to 9p22, and the D9S3 and D9S19 loci have been mapped in this study by in situ hybridization to 9p21 and 9p13, respectively. We hypothesize the existence of one or more tumor suppressor genes on 9p with a role in the development or progression of NSCLC and MM. © 1993 Wiley‐Liss, Inc.
AB - Previously we have reported non‐random cytogenetic abnormalities involving the short arm of chromosome 9 (9p) in the majority of primary non‐small cell lung cancer (NSCLC) patient samples, which indicated loss of DNA sequences. In another lung tumor, pleural malignant mesothelioma (MM), cytogenetic changes also include apparent deletions of 9p. To define the location and extent of deletions of 9p in NSCLC and MM, Southern blot analyses on six NSCLC and five MM cell lines using molecular probes to 9p loci (IFNA, IFNBI, D9S3, and D9S19) were performed, and DNA dosage was determined by densitometry. Our data demonstrated reduced dosage of 9p sequences in three of six NSCLC and four of five MM lines. A homozygous deletion of D9S3 was found in one NSCLC and one MM cell line. The region of common loss overlapped the D9S3 locus and was flanked by the IFNBI and D9S19 loci. IFNBI has previously been localized to 9p22, and the D9S3 and D9S19 loci have been mapped in this study by in situ hybridization to 9p21 and 9p13, respectively. We hypothesize the existence of one or more tumor suppressor genes on 9p with a role in the development or progression of NSCLC and MM. © 1993 Wiley‐Liss, Inc.
UR - http://www.scopus.com/inward/record.url?scp=0027208150&partnerID=8YFLogxK
U2 - 10.1002/gcc.2870070108
DO - 10.1002/gcc.2870070108
M3 - Article
C2 - 7688555
AN - SCOPUS:0027208150
VL - 7
SP - 47
EP - 53
JO - Genes Chromosomes & Cancer
JF - Genes Chromosomes & Cancer
SN - 1045-2257
IS - 1
ER -