Molecular cloning of Quek 1 and 2, two quail vascular endothelial growth factor (VEGF) receptor-like molecules

Anne Eichmann, Christophe Marcelle, Christiane Bréant, Nicole M. Le Douarin

Research output: Contribution to journalArticleResearchpeer-review

Abstract

We have previously reported the cloning of two partial cDNAs corresponding to two quail (Coturnix coturnix japonica) receptor tyrosine kinases (RTKs), named Quek 1 and Quek 2, and their expression in endothelial cells of the early avian embryo. We here report the cloning of the full-size cDNAs for both molecules. Sequence comparison shows that Quek 1 and 2 share an overall amino acid (aa) identity of 49%. They both comprise seven extracellular immunoglobulin-like (Ig-like) domains, a single transmembrane domain, and an intracellular kinase domain split into two by a 70 aa insertion. These structural characteristics are shared by the members of the recently discovered VEGF receptor (VEGFR) family. We have compared the sequences of Quek 1 and 2 to the other VEGFRs. At the aa level, Quek 1 is most closely related to KDR/flk-1 (VEGFR 2) (aa identity of 69% and 71%, respectively). Quek 2 shows a similar degree of aa identity to flt-4 (VEGFR 3). Quek 1 and 2 display a lower homology to flt-1 (VEGFR 1) (about 45% aa identity). These data suggest that Quek 1 and 2 are the avian homologues of VEGFRs 2 and 3, respectively.

Original languageEnglish
Pages (from-to)3-8
Number of pages6
JournalGene
Volume174
Issue number1
DOIs
Publication statusPublished - 26 Sep 1996
Externally publishedYes

Keywords

  • Amino acid sequence
  • Coturnix cotutnix japonica
  • Embryonic development
  • Endothelial cell
  • Receptor tyrosine kinase
  • Recombinant DNA

Cite this

Eichmann, Anne ; Marcelle, Christophe ; Bréant, Christiane ; Le Douarin, Nicole M. / Molecular cloning of Quek 1 and 2, two quail vascular endothelial growth factor (VEGF) receptor-like molecules. In: Gene. 1996 ; Vol. 174, No. 1. pp. 3-8.
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abstract = "We have previously reported the cloning of two partial cDNAs corresponding to two quail (Coturnix coturnix japonica) receptor tyrosine kinases (RTKs), named Quek 1 and Quek 2, and their expression in endothelial cells of the early avian embryo. We here report the cloning of the full-size cDNAs for both molecules. Sequence comparison shows that Quek 1 and 2 share an overall amino acid (aa) identity of 49{\%}. They both comprise seven extracellular immunoglobulin-like (Ig-like) domains, a single transmembrane domain, and an intracellular kinase domain split into two by a 70 aa insertion. These structural characteristics are shared by the members of the recently discovered VEGF receptor (VEGFR) family. We have compared the sequences of Quek 1 and 2 to the other VEGFRs. At the aa level, Quek 1 is most closely related to KDR/flk-1 (VEGFR 2) (aa identity of 69{\%} and 71{\%}, respectively). Quek 2 shows a similar degree of aa identity to flt-4 (VEGFR 3). Quek 1 and 2 display a lower homology to flt-1 (VEGFR 1) (about 45{\%} aa identity). These data suggest that Quek 1 and 2 are the avian homologues of VEGFRs 2 and 3, respectively.",
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Molecular cloning of Quek 1 and 2, two quail vascular endothelial growth factor (VEGF) receptor-like molecules. / Eichmann, Anne; Marcelle, Christophe; Bréant, Christiane; Le Douarin, Nicole M.

In: Gene, Vol. 174, No. 1, 26.09.1996, p. 3-8.

Research output: Contribution to journalArticleResearchpeer-review

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