Molecular characterization of latent GDF8 reveals mechanisms of activation

Ryan G. Walker, Jason C. McCoy, Magdalena Czepnik, Melanie J. Mills, Adam Hagg, Kelly L. Walton, Thomas R. Cotton, Marko Hyvönen, Richard T. Lee, Paul Gregorevic, Craig A. Harrison, Thomas B. Thompson

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain, much like TGF-β. Using a combination of small-angle X-ray scattering and mutagenesis, we characterized the interactions of GDF8 with its prodomain. Our results show that the prodomain:GDF8 complex can exist in a fully latent state and an activated or “triggered” state where the prodomain remains in complex with the mature domain. However, these states are not reversible, indicating the latent GDF8 is “spring-loaded.” Structural analysis shows that the prodomain: GDF8 complex adopts an “open” configuration, distinct from the latency state of TGF-β and more similar to the open state of Activin A and BMP9 (nonlatent complexes). We determined that GDF8 maintains similar features for latency, including the alpha-1 helix and fastener elements, and identified a series of mutations in the prodomain of GDF8 that alleviate latency, including I56E, which does not require activation by the protease Tolloid. In vivo, active GDF8 variants were potent negative regulators of muscle mass, compared with WT GDF8. Collectively, these results help characterize the latency and activation mechanisms of GDF8.

Original languageEnglish
Pages (from-to)E866-E875
Number of pages10
JournalProceedings of the National Academy of Sciences of the United States of America
Volume115
Issue number5
DOIs
Publication statusPublished - 30 Jan 2018

Keywords

  • GDF8
  • GDF8 prodomain
  • latent activation
  • myostatin activation
  • myostatin prodomain

Cite this

Walker, Ryan G. ; McCoy, Jason C. ; Czepnik, Magdalena ; Mills, Melanie J. ; Hagg, Adam ; Walton, Kelly L. ; Cotton, Thomas R. ; Hyvönen, Marko ; Lee, Richard T. ; Gregorevic, Paul ; Harrison, Craig A. ; Thompson, Thomas B. / Molecular characterization of latent GDF8 reveals mechanisms of activation. In: Proceedings of the National Academy of Sciences of the United States of America. 2018 ; Vol. 115, No. 5. pp. E866-E875.
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abstract = "Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain, much like TGF-β. Using a combination of small-angle X-ray scattering and mutagenesis, we characterized the interactions of GDF8 with its prodomain. Our results show that the prodomain:GDF8 complex can exist in a fully latent state and an activated or “triggered” state where the prodomain remains in complex with the mature domain. However, these states are not reversible, indicating the latent GDF8 is “spring-loaded.” Structural analysis shows that the prodomain: GDF8 complex adopts an “open” configuration, distinct from the latency state of TGF-β and more similar to the open state of Activin A and BMP9 (nonlatent complexes). We determined that GDF8 maintains similar features for latency, including the alpha-1 helix and fastener elements, and identified a series of mutations in the prodomain of GDF8 that alleviate latency, including I56E, which does not require activation by the protease Tolloid. In vivo, active GDF8 variants were potent negative regulators of muscle mass, compared with WT GDF8. Collectively, these results help characterize the latency and activation mechanisms of GDF8.",
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Molecular characterization of latent GDF8 reveals mechanisms of activation. / Walker, Ryan G.; McCoy, Jason C.; Czepnik, Magdalena; Mills, Melanie J.; Hagg, Adam; Walton, Kelly L.; Cotton, Thomas R.; Hyvönen, Marko; Lee, Richard T.; Gregorevic, Paul; Harrison, Craig A.; Thompson, Thomas B.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, No. 5, 30.01.2018, p. E866-E875.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Molecular characterization of latent GDF8 reveals mechanisms of activation

AU - Walker, Ryan G.

AU - McCoy, Jason C.

AU - Czepnik, Magdalena

AU - Mills, Melanie J.

AU - Hagg, Adam

AU - Walton, Kelly L.

AU - Cotton, Thomas R.

AU - Hyvönen, Marko

AU - Lee, Richard T.

AU - Gregorevic, Paul

AU - Harrison, Craig A.

AU - Thompson, Thomas B.

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