Molecular characterization and clonal dynamics of nosocomial blaOXA-23 producing XDR Acinetobacter baumannii

Sabrina Royer, Paola Amaral de Campos, Bruna Fuga Araújo, Melina Lorraine Ferreira, Iara Rossi Gonçalves, Deivid William da Fonseca Batistão, Rebecca Tavares e Silva Brígido, Louise Teixeira Cerdeira, Luiz Gustavo Machado, Cristiane Silveira de Brito, Paulo Pinto Gontijo-Filho, Rosineide Marques Ribas

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

The emergence of infections associated to new antimicrobial resistance in Acinetobacter baumannii (Ab) genotypes represents a major challenge. In this context, this study aimed to determine the diversity of resistance mechanisms and investigate clonal dissemination and predominant sequence types (STs) in multidrug-resistant Ab strains of clinical (tracheal aspirate, n = 17) and environmental (surface, n = 6) origins. Additionally, the major clones found in clinical (A) and environmental (H) strains had their complete genomes sequenced. All strains were submitted to polymerase chain reactions (PCR) for the detection of the ISAba1/blaOXA-51-like and ISAba1/blaOXA-23-like genes, while the expression of genes encoding the carO porin, AdeABC (adeB), AdeFGH (adeG), and AdeIJK (adeJ) efflux pumps was determined by real time PCR (qPCR). Most of the strains were characterized as extensively drug-resistant (XDR) with high minimal inhibitory concentrations (MICs) detected for tigecycline and carbapenems. Associations between ISAba1/OXA-51 and ISAba1/OXA-23 were observed in 91.3% and 52.2% of the strains, respectively. Only the adeB gene was considered hyper-expressed. Furthermore, most of the strains analyzed by the MuLtilocus Sequence-Typing (MLST) were found to belong to the clonal complex 113 (CC113). In addition, a new ST, ST1399, belonging to CC229, was also discovered herein. Strains analyzed by whole genome sequencing presented resistance genes linked to multidrug-resistance phenotypes and confirmed the presence of Tn2008, which provides high levels carbapenem-resistance.

Original languageEnglish
Article numbere0198643
Number of pages14
JournalPLoS ONE
Volume13
Issue number6
DOIs
Publication statusPublished - Jun 2018
Externally publishedYes

Cite this

Royer, S., Amaral de Campos, P., Araújo, B. F., Ferreira, M. L., Gonçalves, I. R., William da Fonseca Batistão, D., Tavares e Silva Brígido, R., Cerdeira, L. T., Machado, L. G., Silveira de Brito, C., Gontijo-Filho, P. P., & Ribas, R. M. (2018). Molecular characterization and clonal dynamics of nosocomial blaOXA-23 producing XDR Acinetobacter baumannii. PLoS ONE, 13(6), [e0198643]. https://doi.org/10.1371/journal.pone.0198643