Molecular basis for the nerve dependence of limb regeneration in an adult vertebrate

Anoop Kumar, James Walter Godwin, Phillip B Gates, A Acely Garza-Garcia, Jeremy P Brockes

Research output: Contribution to journalArticleResearchpeer-review

316 Citations (Scopus)

Abstract

The limb blastemal cells of an adult salamander regenerate the structures distal to the level of amputation, and the surface protein Prod 1 is a critical determinant of their proximodistal identity. The anterior gradient protein family member nAG is a secreted ligand for Prod 1 and a growth factor for cultured newt blastemal cells. nAG is sequentially expressed after amputation in the regenerating nerve and the wound epidermis-the key tissues of the stem cell niche-and its expression in both locations is abrogated by denervation. The local expression of nAG after electroporation is sufficient to rescue a denervated blastema and regenerate the distal structures. Our analysis brings together the positional identity of the blastema and the classical nerve dependence of limb regeneration.
Original languageEnglish
Pages (from-to)772 - 777
Number of pages6
JournalScience
Volume318
Issue number5851
DOIs
Publication statusPublished - 2007
Externally publishedYes

Cite this

Kumar, A., Godwin, J. W., Gates, P. B., Garza-Garcia, A. A., & Brockes, J. P. (2007). Molecular basis for the nerve dependence of limb regeneration in an adult vertebrate. Science, 318(5851), 772 - 777. https://doi.org/10.1126/science.1147710