Molecular basis for jagged-1/serrate ligand recognition by the notch receptor

Pat Whiteman; Beatriz Hernandez De Madrid; Paul Taylor; Demin Li; Rebecca Heslop; Nattnee Viticheep; Joyce Zi Tan; Hideyuki Shimizu; Juliana Callaghan; Massimo Masiero; Ji Liang Li; Alison H. Banham; Adrian L Harris; Susan M. Lea; Christina Redfield; Martin Baron; Penny A. Handford

doi:10.1074/jbc.M112.428854

Molecular basis for jagged-1/serrate ligand recognition by the notch receptor

Pat Whiteman, Beatriz Hernandez De Madrid, Paul Taylor, Demin Li, Rebecca Heslop, Nattnee Viticheep, Joyce Zi Tan, Hideyuki Shimizu, Juliana Callaghan, Massimo Masiero, Ji Liang Li, Alison H. Banham, Adrian L Harris, Susan M. Lea, Christina Redfield, Martin Baron, Penny A. Handford

Research output: Contribution to journal › Article › Research › peer-review

26 Citations (Scopus)

Abstract

Background: The site of Jagged/Serrate ligand recognition by Notch is unknown. Results: Two critical residues involved in an intramolecular hydrophobic interaction across the central sheet of EGF12 form a ligand-binding platform. Conclusion: The ligand-binding region is adjacent to a Fringe-sensitive residue involved in modulating Notch activity. Significance: The results have implications for understanding receptor/ligand recognition, Notch regulation by O-glycosylation, and the development of paralogue-specific antibodies.

Original language	English
Pages (from-to)	7305-7312
Number of pages	8
Journal	Journal of Biological Chemistry
Volume	288
Issue number	10
DOIs	https://doi.org/10.1074/jbc.M112.428854
Publication status	Published - 8 Mar 2013
Externally published	Yes

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Whiteman, P., De Madrid, B. H., Taylor, P., Li, D., Heslop, R., Viticheep, N., Tan, J. Z., Shimizu, H., Callaghan, J., Masiero, M., Li, J. L., Banham, A. H., Harris, A. L., Lea, S. M., Redfield, C., Baron, M., & Handford, P. A. (2013). Molecular basis for jagged-1/serrate ligand recognition by the notch receptor. Journal of Biological Chemistry, 288(10), 7305-7312. https://doi.org/10.1074/jbc.M112.428854

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abstract = "Background: The site of Jagged/Serrate ligand recognition by Notch is unknown. Results: Two critical residues involved in an intramolecular hydrophobic interaction across the central sheet of EGF12 form a ligand-binding platform. Conclusion: The ligand-binding region is adjacent to a Fringe-sensitive residue involved in modulating Notch activity. Significance: The results have implications for understanding receptor/ligand recognition, Notch regulation by O-glycosylation, and the development of paralogue-specific antibodies.",

author = "Pat Whiteman and {De Madrid}, {Beatriz Hernandez} and Paul Taylor and Demin Li and Rebecca Heslop and Nattnee Viticheep and Tan, {Joyce Zi} and Hideyuki Shimizu and Juliana Callaghan and Massimo Masiero and Li, {Ji Liang} and Banham, {Alison H.} and Harris, {Adrian L} and Lea, {Susan M.} and Christina Redfield and Martin Baron and Handford, {Penny A.}",

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doi = "10.1074/jbc.M112.428854",

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Whiteman, P, De Madrid, BH, Taylor, P, Li, D, Heslop, R, Viticheep, N, Tan, JZ, Shimizu, H, Callaghan, J, Masiero, M, Li, JL, Banham, AH, Harris, AL, Lea, SM, Redfield, C, Baron, M & Handford, PA 2013, 'Molecular basis for jagged-1/serrate ligand recognition by the notch receptor', Journal of Biological Chemistry, vol. 288, no. 10, pp. 7305-7312. https://doi.org/10.1074/jbc.M112.428854

TY - JOUR

T1 - Molecular basis for jagged-1/serrate ligand recognition by the notch receptor

AU - Whiteman, Pat

AU - De Madrid, Beatriz Hernandez

AU - Taylor, Paul

AU - Li, Demin

AU - Heslop, Rebecca

AU - Viticheep, Nattnee

AU - Tan, Joyce Zi

AU - Shimizu, Hideyuki

AU - Callaghan, Juliana

AU - Masiero, Massimo

AU - Li, Ji Liang

AU - Banham, Alison H.

AU - Harris, Adrian L

AU - Lea, Susan M.

AU - Redfield, Christina

AU - Baron, Martin

AU - Handford, Penny A.

PY - 2013/3/8

Y1 - 2013/3/8

N2 - Background: The site of Jagged/Serrate ligand recognition by Notch is unknown. Results: Two critical residues involved in an intramolecular hydrophobic interaction across the central sheet of EGF12 form a ligand-binding platform. Conclusion: The ligand-binding region is adjacent to a Fringe-sensitive residue involved in modulating Notch activity. Significance: The results have implications for understanding receptor/ligand recognition, Notch regulation by O-glycosylation, and the development of paralogue-specific antibodies.

AB - Background: The site of Jagged/Serrate ligand recognition by Notch is unknown. Results: Two critical residues involved in an intramolecular hydrophobic interaction across the central sheet of EGF12 form a ligand-binding platform. Conclusion: The ligand-binding region is adjacent to a Fringe-sensitive residue involved in modulating Notch activity. Significance: The results have implications for understanding receptor/ligand recognition, Notch regulation by O-glycosylation, and the development of paralogue-specific antibodies.

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U2 - 10.1074/jbc.M112.428854

DO - 10.1074/jbc.M112.428854

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AN - SCOPUS:84874859628

SN - 0021-9258

VL - 288

SP - 7305

EP - 7312

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 10

ER -

Molecular basis for jagged-1/serrate ligand recognition by the notch receptor

Abstract

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