Molecular basis for jagged-1/serrate ligand recognition by the notch receptor

Pat Whiteman, Beatriz Hernandez De Madrid, Paul Taylor, Demin Li, Rebecca Heslop, Nattnee Viticheep, Joyce Zi Tan, Hideyuki Shimizu, Juliana Callaghan, Massimo Masiero, Ji Liang Li, Alison H. Banham, Adrian L Harris, Susan M. Lea, Christina Redfield, Martin Baron, Penny A. Handford

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21 Citations (Scopus)

Abstract

Background: The site of Jagged/Serrate ligand recognition by Notch is unknown. Results: Two critical residues involved in an intramolecular hydrophobic interaction across the central sheet of EGF12 form a ligand-binding platform. Conclusion: The ligand-binding region is adjacent to a Fringe-sensitive residue involved in modulating Notch activity. Significance: The results have implications for understanding receptor/ligand recognition, Notch regulation by O-glycosylation, and the development of paralogue-specific antibodies.

Original languageEnglish
Pages (from-to)7305-7312
Number of pages8
JournalThe Journal of Biological Chemistry
Volume288
Issue number10
DOIs
Publication statusPublished - 8 Mar 2013
Externally publishedYes

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