Molecular architecture of the alphabeta T cell receptor-CD3 complex

Michael E Birnbaum, Richard Berry, Yu-Shan Stephanie Hsiao, Zhenjun Chen, Miguel A Shingu-Vazquez, Xiaoling Yu, Deepa Waghray, Suzanne Fischer, James McCluskey, Jamie Rossjohn, Thomas Walz, K Christopher Garcia

Research output: Contribution to journalArticleResearchpeer-review

47 Citations (Scopus)

Abstract

alphabeta T-cell receptor (TCR) activation plays a crucial role for T-cell function. However, the TCR itself does not possess signaling domains. Instead, the TCR is noncovalently coupled to a conserved multisubunit signaling apparatus, the CD3 complex, that comprises the CD3epsilongamma, CD3epsilondelta, and CD3zetazeta dimers. How antigen ligation by the TCR triggers CD3 activation and what structural role the CD3 extracellular domains (ECDs) play in the assembled TCR-CD3 complex remain unclear. Here, we use two complementary structural approaches to gain insight into the overall organization of the TCR-CD3 complex. Small-angle X-ray scattering of the soluble TCR-CD3epsilondelta complex reveals the CD3epsilondelta ECDs to sit underneath the TCR alpha-chain. The observed arrangement is consistent with EM images of the entire TCR-CD3 integral membrane complex, in which the CD3epsilondelta and CD3epsilongamma subunits were situated underneath the TCR alpha-chain and TCR beta-chain, respectively. Interestingly, the TCR-CD3 transmembrane complex bound to peptide-MHC is a dimer in which two TCRs project outward from a central core composed of the CD3 ECDs and the TCR and CD3 transmembrane domains. This arrangement suggests a potential ligand-dependent dimerization mechanism for TCR signaling. Collectively, our data advance our understanding of the molecular organization of the TCR-CD3 complex, and provides a conceptual framework for the TCR activation mechanism.
Original languageEnglish
Pages (from-to)17576 - 17581
Number of pages6
JournalProceedings of the National Academy of Sciences
Volume111
Issue number49
DOIs
Publication statusPublished - 2014

Cite this

Birnbaum, M. E., Berry, R., Hsiao, Y-S. S., Chen, Z., Shingu-Vazquez, M. A., Yu, X., ... Garcia, K. C. (2014). Molecular architecture of the alphabeta T cell receptor-CD3 complex. Proceedings of the National Academy of Sciences, 111(49), 17576 - 17581. https://doi.org/10.1073/pnas.1420936111
Birnbaum, Michael E ; Berry, Richard ; Hsiao, Yu-Shan Stephanie ; Chen, Zhenjun ; Shingu-Vazquez, Miguel A ; Yu, Xiaoling ; Waghray, Deepa ; Fischer, Suzanne ; McCluskey, James ; Rossjohn, Jamie ; Walz, Thomas ; Garcia, K Christopher. / Molecular architecture of the alphabeta T cell receptor-CD3 complex. In: Proceedings of the National Academy of Sciences. 2014 ; Vol. 111, No. 49. pp. 17576 - 17581.
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abstract = "alphabeta T-cell receptor (TCR) activation plays a crucial role for T-cell function. However, the TCR itself does not possess signaling domains. Instead, the TCR is noncovalently coupled to a conserved multisubunit signaling apparatus, the CD3 complex, that comprises the CD3epsilongamma, CD3epsilondelta, and CD3zetazeta dimers. How antigen ligation by the TCR triggers CD3 activation and what structural role the CD3 extracellular domains (ECDs) play in the assembled TCR-CD3 complex remain unclear. Here, we use two complementary structural approaches to gain insight into the overall organization of the TCR-CD3 complex. Small-angle X-ray scattering of the soluble TCR-CD3epsilondelta complex reveals the CD3epsilondelta ECDs to sit underneath the TCR alpha-chain. The observed arrangement is consistent with EM images of the entire TCR-CD3 integral membrane complex, in which the CD3epsilondelta and CD3epsilongamma subunits were situated underneath the TCR alpha-chain and TCR beta-chain, respectively. Interestingly, the TCR-CD3 transmembrane complex bound to peptide-MHC is a dimer in which two TCRs project outward from a central core composed of the CD3 ECDs and the TCR and CD3 transmembrane domains. This arrangement suggests a potential ligand-dependent dimerization mechanism for TCR signaling. Collectively, our data advance our understanding of the molecular organization of the TCR-CD3 complex, and provides a conceptual framework for the TCR activation mechanism.",
author = "Birnbaum, {Michael E} and Richard Berry and Hsiao, {Yu-Shan Stephanie} and Zhenjun Chen and Shingu-Vazquez, {Miguel A} and Xiaoling Yu and Deepa Waghray and Suzanne Fischer and James McCluskey and Jamie Rossjohn and Thomas Walz and Garcia, {K Christopher}",
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Birnbaum, ME, Berry, R, Hsiao, Y-SS, Chen, Z, Shingu-Vazquez, MA, Yu, X, Waghray, D, Fischer, S, McCluskey, J, Rossjohn, J, Walz, T & Garcia, KC 2014, 'Molecular architecture of the alphabeta T cell receptor-CD3 complex', Proceedings of the National Academy of Sciences, vol. 111, no. 49, pp. 17576 - 17581. https://doi.org/10.1073/pnas.1420936111

Molecular architecture of the alphabeta T cell receptor-CD3 complex. / Birnbaum, Michael E; Berry, Richard; Hsiao, Yu-Shan Stephanie; Chen, Zhenjun; Shingu-Vazquez, Miguel A; Yu, Xiaoling; Waghray, Deepa; Fischer, Suzanne; McCluskey, James; Rossjohn, Jamie; Walz, Thomas; Garcia, K Christopher.

In: Proceedings of the National Academy of Sciences, Vol. 111, No. 49, 2014, p. 17576 - 17581.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Molecular architecture of the alphabeta T cell receptor-CD3 complex

AU - Birnbaum, Michael E

AU - Berry, Richard

AU - Hsiao, Yu-Shan Stephanie

AU - Chen, Zhenjun

AU - Shingu-Vazquez, Miguel A

AU - Yu, Xiaoling

AU - Waghray, Deepa

AU - Fischer, Suzanne

AU - McCluskey, James

AU - Rossjohn, Jamie

AU - Walz, Thomas

AU - Garcia, K Christopher

PY - 2014

Y1 - 2014

N2 - alphabeta T-cell receptor (TCR) activation plays a crucial role for T-cell function. However, the TCR itself does not possess signaling domains. Instead, the TCR is noncovalently coupled to a conserved multisubunit signaling apparatus, the CD3 complex, that comprises the CD3epsilongamma, CD3epsilondelta, and CD3zetazeta dimers. How antigen ligation by the TCR triggers CD3 activation and what structural role the CD3 extracellular domains (ECDs) play in the assembled TCR-CD3 complex remain unclear. Here, we use two complementary structural approaches to gain insight into the overall organization of the TCR-CD3 complex. Small-angle X-ray scattering of the soluble TCR-CD3epsilondelta complex reveals the CD3epsilondelta ECDs to sit underneath the TCR alpha-chain. The observed arrangement is consistent with EM images of the entire TCR-CD3 integral membrane complex, in which the CD3epsilondelta and CD3epsilongamma subunits were situated underneath the TCR alpha-chain and TCR beta-chain, respectively. Interestingly, the TCR-CD3 transmembrane complex bound to peptide-MHC is a dimer in which two TCRs project outward from a central core composed of the CD3 ECDs and the TCR and CD3 transmembrane domains. This arrangement suggests a potential ligand-dependent dimerization mechanism for TCR signaling. Collectively, our data advance our understanding of the molecular organization of the TCR-CD3 complex, and provides a conceptual framework for the TCR activation mechanism.

AB - alphabeta T-cell receptor (TCR) activation plays a crucial role for T-cell function. However, the TCR itself does not possess signaling domains. Instead, the TCR is noncovalently coupled to a conserved multisubunit signaling apparatus, the CD3 complex, that comprises the CD3epsilongamma, CD3epsilondelta, and CD3zetazeta dimers. How antigen ligation by the TCR triggers CD3 activation and what structural role the CD3 extracellular domains (ECDs) play in the assembled TCR-CD3 complex remain unclear. Here, we use two complementary structural approaches to gain insight into the overall organization of the TCR-CD3 complex. Small-angle X-ray scattering of the soluble TCR-CD3epsilondelta complex reveals the CD3epsilondelta ECDs to sit underneath the TCR alpha-chain. The observed arrangement is consistent with EM images of the entire TCR-CD3 integral membrane complex, in which the CD3epsilondelta and CD3epsilongamma subunits were situated underneath the TCR alpha-chain and TCR beta-chain, respectively. Interestingly, the TCR-CD3 transmembrane complex bound to peptide-MHC is a dimer in which two TCRs project outward from a central core composed of the CD3 ECDs and the TCR and CD3 transmembrane domains. This arrangement suggests a potential ligand-dependent dimerization mechanism for TCR signaling. Collectively, our data advance our understanding of the molecular organization of the TCR-CD3 complex, and provides a conceptual framework for the TCR activation mechanism.

UR - http://www.pnas.org/content/111/49/17576.full.pdf+html

U2 - 10.1073/pnas.1420936111

DO - 10.1073/pnas.1420936111

M3 - Article

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SP - 17576

EP - 17581

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

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