Molecular and physiological analysis of three Pseudomonas aeruginosa phages belonging to the "N4-like viruses"

Pieter Jan Ceyssens, Andrew Brabban, Larissa Rogge, Matthew Spooner Lewis, Derek Pickard, David Goulding, Gordon Dougan, Jean Paul Noben, Andrew Kropinski, Elizabeth Kutter, Rob Lavigne

Research output: Contribution to journalArticleResearchpeer-review

46 Citations (Scopus)

Abstract

We present a detailed analysis of the genome architecture, structural proteome and infection-related properties of three Pseudomonas phages, designated LUZ7, LIT1 and PEV2. These podoviruses encapsulate 72.5 to 74.9. kb genomes and lyse their host after 25. min aerobic infection. PEV2 can successfully infect under anaerobic conditions, but its latent period is tripled, the lysis proceeds far slower and the burst size decreases significantly. While the overall genome structure of these phages resembles the well-studied coliphage N4, these Pseudomonas phages encode a cluster of tail genes which displays significant similarity to a Pseudomonas aeruginosa (cryptic) prophage region. Using ESI-MS/MS, these tail proteins were shown to be part of the phage particle, as well as ten other proteins including a giant 370. kDa virion RNA polymerase. These phages are the first described representatives of a novel kind of obligatory lytic P. aeruginosa-infecting phages, belonging to the widespread "N4-like viruses" genus.

Original languageEnglish
Pages (from-to)26-30
Number of pages5
JournalVirology
Volume405
Issue number1
DOIs
Publication statusPublished - 1 Sep 2010
Externally publishedYes

Keywords

  • Anaerobic infection
  • Bacteriophage
  • Genomics
  • Mass spectrometry
  • Phage N4
  • Pseudomonas aeruginosa

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