TY - JOUR
T1 - Molecular analysis of a Leptospira borgpetersenii gene encoding an endoflagellar subunit protein
AU - Harper (Mitchison), Marina
AU - Rood, J. I.
AU - Faine, S.
AU - Adler, B.
PY - 1991
Y1 - 1991
N2 - A flagellin gene, flaB, from Leptospira borgpetersenii (formerly L. interrogans) serovar hardjo was cloned and expressed in Escherichia coli. Expression of the 32 kDa FlaB protein was dependent upon the lacZ promoter from pUC18. Nucleotide sequence data showed an open reading frame encoding 283 amino acid residues, corresponding to a protein of molecular mass 31.3 kDa. The G + C content of the flaB gene was 54.7 mol%. Comparison of the deduced FlaB amino acid sequence with flagellins from other bacteria revealed a high level of identity with the Treponema pallidum FlaB proteins.
AB - A flagellin gene, flaB, from Leptospira borgpetersenii (formerly L. interrogans) serovar hardjo was cloned and expressed in Escherichia coli. Expression of the 32 kDa FlaB protein was dependent upon the lacZ promoter from pUC18. Nucleotide sequence data showed an open reading frame encoding 283 amino acid residues, corresponding to a protein of molecular mass 31.3 kDa. The G + C content of the flaB gene was 54.7 mol%. Comparison of the deduced FlaB amino acid sequence with flagellins from other bacteria revealed a high level of identity with the Treponema pallidum FlaB proteins.
UR - http://www.scopus.com/inward/record.url?scp=0025733914&partnerID=8YFLogxK
U2 - 10.1099/00221287-137-7-1529
DO - 10.1099/00221287-137-7-1529
M3 - Article
C2 - 1955849
AN - SCOPUS:0025733914
SN - 0022-1287
VL - 137
SP - 1529
EP - 1536
JO - Journal of General Microbiology
JF - Journal of General Microbiology
IS - 7
ER -