The human neuroblastoma cell line T98G is sensitive to the antiproliferative activity of both α- and γ-interferons (IFNs). Specific binding of a homogeneous 125I-IFN-α2 probe to high-affinity membrane receptors (apparent K(d) = 6.2 x 10-10 M) can be decreased by pretreatment of these cells with human IFN-γ (HuIFN-γ, derived by recombinant DNA techniques). The regulation of HuIFN-α2 binding by such pretreatment is reflected by subsequent inhibition of both the antiviral and antiproliferative activities of HuIFN-α2 on T98G cells. Thus, binding of HuIFN-γ to its distinct cell-surface receptor can modify expression of the separate IFN-α receptor, presumably through an indirect effect on the apparent K(d) of the IFN-α2 receptor-ligand interaction.
|Number of pages||3|
|Journal||The Journal of Biological Chemistry|
|Publication status||Published - 1 Jan 1984|