Modulation of human interferon-α receptor expression by human interferon-γ

G. E. Hannigan, E. N. Fish, B. R.G. Williams

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The human neuroblastoma cell line T98G is sensitive to the antiproliferative activity of both α- and γ-interferons (IFNs). Specific binding of a homogeneous 125I-IFN-α2 probe to high-affinity membrane receptors (apparent K(d) = 6.2 x 10-10 M) can be decreased by pretreatment of these cells with human IFN-γ (HuIFN-γ, derived by recombinant DNA techniques). The regulation of HuIFN-α2 binding by such pretreatment is reflected by subsequent inhibition of both the antiviral and antiproliferative activities of HuIFN-α2 on T98G cells. Thus, binding of HuIFN-γ to its distinct cell-surface receptor can modify expression of the separate IFN-α receptor, presumably through an indirect effect on the apparent K(d) of the IFN-α2 receptor-ligand interaction.

Original languageEnglish
Pages (from-to)8084-8086
Number of pages3
JournalThe Journal of Biological Chemistry
Issue number13
Publication statusPublished - 1 Jan 1984
Externally publishedYes

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