Modulation of fetal inflammatory response on exposure to lipopolysaccharide by chorioamnion, lung, or gut in sheep

Boris W Kramer, Suhas G Kallapur, Timothy J M Moss, Ilias Nitsos, Graeme P Polglase, John P Newnham, Alan H Jobe

Research output: Contribution to journalArticleResearchpeer-review

Abstract

OBJECTIVE: We hypothesized that fetal lipopolysaccharide exposures to the chorioamnion, lung, or gut would induce distinct systemic inflammatory responses. STUDY DESIGN: Groups of 5-7 time-mated ewes were used to surgically isolate the fetal respiratory and the gastrointestinal systems from the amniotic compartment. Outcomes were assessed at 124 days gestational age, 2 days and 7 days after lipopolysaccharide (10 mg, Escherichia coli 055:B5) or saline solution infusions into the fetal airways or amniotic fluid. RESULTS: Lipopolysaccharide induced systemic inflammatory changes in all groups in the blood, lung, liver, and thymic lymphocytes. Changes in lymphocytes in the posterior mediastinal lymph node draining lung and gut, occurred only after direct contact of lipopolysaccharide with the fetal lung or gut. CONCLUSION: Fetal systemic inflammatory responses occurred after chorioamnion, lung, or gut exposures to lipopolysaccharide. The organ responses differed based on route of the fetal exposure.
Original languageEnglish
Pages (from-to)77.e1 - 77.e9
Number of pages9
JournalAmerican Journal of Obstetrics and Gynecology
Volume202
Issue number1
DOIs
Publication statusPublished - 2010

Cite this

Kramer, Boris W ; Kallapur, Suhas G ; Moss, Timothy J M ; Nitsos, Ilias ; Polglase, Graeme P ; Newnham, John P ; Jobe, Alan H. / Modulation of fetal inflammatory response on exposure to lipopolysaccharide by chorioamnion, lung, or gut in sheep. In: American Journal of Obstetrics and Gynecology. 2010 ; Vol. 202, No. 1. pp. 77.e1 - 77.e9.
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title = "Modulation of fetal inflammatory response on exposure to lipopolysaccharide by chorioamnion, lung, or gut in sheep",
abstract = "OBJECTIVE: We hypothesized that fetal lipopolysaccharide exposures to the chorioamnion, lung, or gut would induce distinct systemic inflammatory responses. STUDY DESIGN: Groups of 5-7 time-mated ewes were used to surgically isolate the fetal respiratory and the gastrointestinal systems from the amniotic compartment. Outcomes were assessed at 124 days gestational age, 2 days and 7 days after lipopolysaccharide (10 mg, Escherichia coli 055:B5) or saline solution infusions into the fetal airways or amniotic fluid. RESULTS: Lipopolysaccharide induced systemic inflammatory changes in all groups in the blood, lung, liver, and thymic lymphocytes. Changes in lymphocytes in the posterior mediastinal lymph node draining lung and gut, occurred only after direct contact of lipopolysaccharide with the fetal lung or gut. CONCLUSION: Fetal systemic inflammatory responses occurred after chorioamnion, lung, or gut exposures to lipopolysaccharide. The organ responses differed based on route of the fetal exposure.",
author = "Kramer, {Boris W} and Kallapur, {Suhas G} and Moss, {Timothy J M} and Ilias Nitsos and Polglase, {Graeme P} and Newnham, {John P} and Jobe, {Alan H}",
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doi = "10.1016/j.ajog.2009.07.058",
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Modulation of fetal inflammatory response on exposure to lipopolysaccharide by chorioamnion, lung, or gut in sheep. / Kramer, Boris W; Kallapur, Suhas G; Moss, Timothy J M; Nitsos, Ilias; Polglase, Graeme P; Newnham, John P; Jobe, Alan H.

In: American Journal of Obstetrics and Gynecology, Vol. 202, No. 1, 2010, p. 77.e1 - 77.e9.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Modulation of fetal inflammatory response on exposure to lipopolysaccharide by chorioamnion, lung, or gut in sheep

AU - Kramer, Boris W

AU - Kallapur, Suhas G

AU - Moss, Timothy J M

AU - Nitsos, Ilias

AU - Polglase, Graeme P

AU - Newnham, John P

AU - Jobe, Alan H

PY - 2010

Y1 - 2010

N2 - OBJECTIVE: We hypothesized that fetal lipopolysaccharide exposures to the chorioamnion, lung, or gut would induce distinct systemic inflammatory responses. STUDY DESIGN: Groups of 5-7 time-mated ewes were used to surgically isolate the fetal respiratory and the gastrointestinal systems from the amniotic compartment. Outcomes were assessed at 124 days gestational age, 2 days and 7 days after lipopolysaccharide (10 mg, Escherichia coli 055:B5) or saline solution infusions into the fetal airways or amniotic fluid. RESULTS: Lipopolysaccharide induced systemic inflammatory changes in all groups in the blood, lung, liver, and thymic lymphocytes. Changes in lymphocytes in the posterior mediastinal lymph node draining lung and gut, occurred only after direct contact of lipopolysaccharide with the fetal lung or gut. CONCLUSION: Fetal systemic inflammatory responses occurred after chorioamnion, lung, or gut exposures to lipopolysaccharide. The organ responses differed based on route of the fetal exposure.

AB - OBJECTIVE: We hypothesized that fetal lipopolysaccharide exposures to the chorioamnion, lung, or gut would induce distinct systemic inflammatory responses. STUDY DESIGN: Groups of 5-7 time-mated ewes were used to surgically isolate the fetal respiratory and the gastrointestinal systems from the amniotic compartment. Outcomes were assessed at 124 days gestational age, 2 days and 7 days after lipopolysaccharide (10 mg, Escherichia coli 055:B5) or saline solution infusions into the fetal airways or amniotic fluid. RESULTS: Lipopolysaccharide induced systemic inflammatory changes in all groups in the blood, lung, liver, and thymic lymphocytes. Changes in lymphocytes in the posterior mediastinal lymph node draining lung and gut, occurred only after direct contact of lipopolysaccharide with the fetal lung or gut. CONCLUSION: Fetal systemic inflammatory responses occurred after chorioamnion, lung, or gut exposures to lipopolysaccharide. The organ responses differed based on route of the fetal exposure.

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