Abstract
Collagen II, a major extracellular matrix component in cartilaginous tissues, undergoes fibrillogenesis under physiological conditions. The present study explored collagen II fiber formation in solution and in two- (coverslip) and three-dimensional (scaffold) environments under different incubation conditions. These conditions include variations in adsorption buffers, the presence of 1-ethyl-3-(3-dimenthylaminopropyl) carbodiimide/N-hydroxysuccinimide crosslinker and the nature of the material surfaces. We extend our observations of collagen II fiber formation in two dimensions to develop an approach for the formation of a fibrillar collagen II network throughout surface-modified polylactide-co-glycolide porous scaffolds. Morphologically, the collagen II network is similar to that present in native articular cartilage. Biological validation of the resultant optimized functional scaffold, using rat bone marrow-derived mesenchymal stem cells, shows appreciable cell infiltration throughout the scaffold with enhanced cell spreading at 24 h post-seeding. This economic and versatile approach is thus believed to have significant potential in cartilage tissue engineering applications.
Original language | English |
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Pages (from-to) | 2804-2816 |
Number of pages | 13 |
Journal | Acta Biomaterialia |
Volume | 7 |
Issue number | 7 |
DOIs | |
Publication status | Published - Jul 2011 |
Externally published | Yes |
Keywords
- 2-D and 3-D environments
- Collagen II
- EDAC/NHS crosslinking
- Fibrillogenesis
- Surface modification