Modulation of collagen II fiber formation in 3-D porous scaffold environments

Guak Kim Tan, Donna Lee M Dinnes, Justin J. Cooper-White

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)

Abstract

Collagen II, a major extracellular matrix component in cartilaginous tissues, undergoes fibrillogenesis under physiological conditions. The present study explored collagen II fiber formation in solution and in two- (coverslip) and three-dimensional (scaffold) environments under different incubation conditions. These conditions include variations in adsorption buffers, the presence of 1-ethyl-3-(3-dimenthylaminopropyl) carbodiimide/N-hydroxysuccinimide crosslinker and the nature of the material surfaces. We extend our observations of collagen II fiber formation in two dimensions to develop an approach for the formation of a fibrillar collagen II network throughout surface-modified polylactide-co-glycolide porous scaffolds. Morphologically, the collagen II network is similar to that present in native articular cartilage. Biological validation of the resultant optimized functional scaffold, using rat bone marrow-derived mesenchymal stem cells, shows appreciable cell infiltration throughout the scaffold with enhanced cell spreading at 24 h post-seeding. This economic and versatile approach is thus believed to have significant potential in cartilage tissue engineering applications. 

Original languageEnglish
Pages (from-to)2804-2816
Number of pages13
JournalActa Biomaterialia
Volume7
Issue number7
DOIs
Publication statusPublished - Jul 2011
Externally publishedYes

Keywords

  • 2-D and 3-D environments
  • Collagen II
  • EDAC/NHS crosslinking
  • Fibrillogenesis
  • Surface modification

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