Modification of carbon nanotubes for gene delivery vectors

Victor Ramos-Perez; Anna Cifuentes Rius; Nuria Coronas; Ana de Pablo; Salvador Borros

doi:10.1007/978-1-62703-462-3_20

Modification of carbon nanotubes for gene delivery vectors

Victor Ramos-Perez, Anna Cifuentes Rius, Nuria Coronas, Ana de Pablo, Salvador Borros

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

17 Citations (Scopus)

Abstract

The surface modification of carbon nanotubes (CNTs) can be tailored to allow the formation of a complex between these potential carriers with DNA. In this chapter, protocols developed in our lab to prepare transfection vectors through the modification of MWCNTs are described. The protocol includes sections focused on the reduction of CNTs length, protocols to increase the dispersability of CNTs and finally, protocols for surface modification to attach through electrostatic interactions DNA to the CNTs.

Original language	English
Title of host publication	Nanomaterial Interfaces in Biology
Subtitle of host publication	Methods and Protocols
Editors	Paolo Bergese, Kimberly Hamad-Schifferli
Publisher	Humana Press
Pages	261-268
Number of pages	8
Volume	1025
ISBN (Electronic)	9781627034623
ISBN (Print)	9781627034616
DOIs	https://doi.org/10.1007/978-1-62703-462-3_20
Publication status	Published - 6 Jul 2013
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press
Volume	1025
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Carbon nanotubes
DNA delivery
Plasma polymerization

Access to Document

10.1007/978-1-62703-462-3_20

Link to publication in Scopus

Cite this

Ramos-Perez, V., Cifuentes Rius, A., Coronas, N., de Pablo, A., & Borros, S. (2013). Modification of carbon nanotubes for gene delivery vectors. In P. Bergese, & K. Hamad-Schifferli (Eds.), Nanomaterial Interfaces in Biology: Methods and Protocols (Vol. 1025, pp. 261-268). (Methods in Molecular Biology; Vol. 1025). Humana Press. https://doi.org/10.1007/978-1-62703-462-3_20

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Modification of carbon nanotubes for gene delivery vectors. / Ramos-Perez, Victor; Cifuentes Rius, Anna; Coronas, Nuria; de Pablo, Ana; Borros, Salvador.

Nanomaterial Interfaces in Biology: Methods and Protocols. ed. / Paolo Bergese; Kimberly Hamad-Schifferli. Vol. 1025 Humana Press, 2013. p. 261-268 (Methods in Molecular Biology; Vol. 1025).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

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