Modification of carbon nanotubes for gene delivery vectors

Victor Ramos-Perez, Anna Cifuentes Rius, Nuria Coronas, Ana de Pablo, Salvador Borros

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

17 Citations (Scopus)

Abstract

The surface modification of carbon nanotubes (CNTs) can be tailored to allow the formation of a complex between these potential carriers with DNA. In this chapter, protocols developed in our lab to prepare transfection vectors through the modification of MWCNTs are described. The protocol includes sections focused on the reduction of CNTs length, protocols to increase the dispersability of CNTs and finally, protocols for surface modification to attach through electrostatic interactions DNA to the CNTs.
Original languageEnglish
Title of host publicationNanomaterial Interfaces in Biology
Subtitle of host publicationMethods and Protocols
EditorsPaolo Bergese, Kimberly Hamad-Schifferli
PublisherHumana Press
Pages261-268
Number of pages8
Volume1025
ISBN (Electronic)9781627034623
ISBN (Print)9781627034616
DOIs
Publication statusPublished - 6 Jul 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1025
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Carbon nanotubes
  • DNA delivery
  • Plasma polymerization

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