Modelling the transport of momentum and oxygen in an aerial-disk driven bioreactor used for animal tissue or cell culture

K. Y S Liow, G. A. Thouas, B. T. Tan, M. C. Thompson, K. Hourigan

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearchpeer-review

12 Citations (Scopus)

Abstract

This study considers the momentum transport and oxygen transfer in a modified stirred tank bioreactor. The design is novel in the sense that the impeller is positioned above the culture medium (instead of being suspended inside it). This design has potential benefits of enhanced gas transfer, reduced possibility of contamination, and better access to the culture medium. Computational fluid dynamics modelling is used to simulate the gas and fluid flow in the bioreactor. A rotation rate of 60 to 240 rpm (corresponding to the laminar regime) was adopted. Results show that the flow in the medium is swirl-dominant with an induced secondary flow in the meridional plane consisting of a steady and robust recirculation bubble. As the Reynolds number is increased beyond ~427, we observe the formation of an additional smaller toroidal-bubble at the bottom wall. This bubble bears some resemblance to the vortex breakdown topology commonly found in confined swirling flows. In terms of the oxygen distribution, oxygen transfer from the gaseous phase into the culture medium is enhanced through forced diffusion taking place across the air-medium interface. For the Reynolds number range studied there is clear dominance of convection over diffusion in the transport of oxygen from the air-medium interface and throughout the culture medium.

Original languageEnglish
Title of host publication13th International Conference on Biomedical Engineering - ICBME 2008
Pages1672-1675
Number of pages4
Volume23
DOIs
Publication statusPublished - 2009
EventInternational Conference on Biomedical Engineering (ICBME) 2008 - Singapore, Singapore
Duration: 3 Dec 20086 Dec 2008
Conference number: 13th

Conference

ConferenceInternational Conference on Biomedical Engineering (ICBME) 2008
Abbreviated titleICBME 2008
CountrySingapore
CitySingapore
Period3/12/086/12/08

Keywords

  • Bioreactor
  • Computational fluid dynamics
  • Oxygen concentration
  • Tissue engineering

Cite this

Liow, K. Y. S., Thouas, G. A., Tan, B. T., Thompson, M. C., & Hourigan, K. (2009). Modelling the transport of momentum and oxygen in an aerial-disk driven bioreactor used for animal tissue or cell culture. In 13th International Conference on Biomedical Engineering - ICBME 2008 (Vol. 23, pp. 1672-1675) https://doi.org/10.1007/978-3-540-92841-6_415
Liow, K. Y S ; Thouas, G. A. ; Tan, B. T. ; Thompson, M. C. ; Hourigan, K. / Modelling the transport of momentum and oxygen in an aerial-disk driven bioreactor used for animal tissue or cell culture. 13th International Conference on Biomedical Engineering - ICBME 2008. Vol. 23 2009. pp. 1672-1675
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Liow, KYS, Thouas, GA, Tan, BT, Thompson, MC & Hourigan, K 2009, Modelling the transport of momentum and oxygen in an aerial-disk driven bioreactor used for animal tissue or cell culture. in 13th International Conference on Biomedical Engineering - ICBME 2008. vol. 23, pp. 1672-1675, International Conference on Biomedical Engineering (ICBME) 2008, Singapore, Singapore, 3/12/08. https://doi.org/10.1007/978-3-540-92841-6_415

Modelling the transport of momentum and oxygen in an aerial-disk driven bioreactor used for animal tissue or cell culture. / Liow, K. Y S; Thouas, G. A.; Tan, B. T.; Thompson, M. C.; Hourigan, K.

13th International Conference on Biomedical Engineering - ICBME 2008. Vol. 23 2009. p. 1672-1675.

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearchpeer-review

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AB - This study considers the momentum transport and oxygen transfer in a modified stirred tank bioreactor. The design is novel in the sense that the impeller is positioned above the culture medium (instead of being suspended inside it). This design has potential benefits of enhanced gas transfer, reduced possibility of contamination, and better access to the culture medium. Computational fluid dynamics modelling is used to simulate the gas and fluid flow in the bioreactor. A rotation rate of 60 to 240 rpm (corresponding to the laminar regime) was adopted. Results show that the flow in the medium is swirl-dominant with an induced secondary flow in the meridional plane consisting of a steady and robust recirculation bubble. As the Reynolds number is increased beyond ~427, we observe the formation of an additional smaller toroidal-bubble at the bottom wall. This bubble bears some resemblance to the vortex breakdown topology commonly found in confined swirling flows. In terms of the oxygen distribution, oxygen transfer from the gaseous phase into the culture medium is enhanced through forced diffusion taking place across the air-medium interface. For the Reynolds number range studied there is clear dominance of convection over diffusion in the transport of oxygen from the air-medium interface and throughout the culture medium.

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Liow KYS, Thouas GA, Tan BT, Thompson MC, Hourigan K. Modelling the transport of momentum and oxygen in an aerial-disk driven bioreactor used for animal tissue or cell culture. In 13th International Conference on Biomedical Engineering - ICBME 2008. Vol. 23. 2009. p. 1672-1675 https://doi.org/10.1007/978-3-540-92841-6_415