Modeling acute myeloid leukemia using StarPEG-heparin hydrogels

P. Lewen Holloway, Akhilandeshwari Ravichandran, Julien Clegg, Claudia Bruedigam, Laura J. Bray

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

Biomimetic semi-synthetic hydrogels formed from a combination of star-shaped poly(ethylene glycol) (starPEG) and the glycosaminoglycan, heparin, allows for the three-dimensional (3D) culture of various cells and tissues. In this chapter, we describe methods for the use of starPEG-heparin hydrogels to cultivate primary and immortalized human acute myeloid leukemia (AML) cells. The resulting 3D culture models allow for the study of AML development and response to chemotherapeutic agents.

Original languageEnglish
Title of host publication3D Cell Culture
Subtitle of host publicationMethods and Protocols
EditorsZuzana Sumbalova Koledova
Place of PublicationNew York NY USA
PublisherSpringer Science + Business Media
Chapter6
Pages61-73
Number of pages13
Volume2764
ISBN (Electronic)9781071636749
ISBN (Print)9781071636732
DOIs
Publication statusPublished - 2024
Externally publishedYes

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume2764
ISSN (Print)1064-3745

Keywords

  • 3D model
  • Acute myeloid leukemia
  • Hydrogel

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