Mobilization of the escherichia coli plasmid colEl (colicin E1) and colel vectors used in recombinant DNA experiments

Gordon Dougan, J. H. Crosa, Stanley Falkow

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The Escherichia coli ColE1 plasmid, which codes for production of colicin E1, is inherently nontransferable (nonconjugative) by bacterial mating. ColE1 can be transmitted at mating by a process called mobilization if ColE1 is coresident with a transfer plasmid. Mobilization is governed in part by a ColE1 gene called mob. ColE1 is mob+. Several ColE1 derivatives employed in recombinant DNA experiments, notably pBR313 and pBR322, are mob-. These cloning vehicles are mobilized at a markedly reduced frequency relative to ColE1. E. coli K12 carrying either pBR313 or pBR322 represents a useful host vector system for recombinant DNA experiments and affords a significant degree of biological containment.

Original languageEnglish
Pages (from-to)676-680
Number of pages5
JournalThe Journal of Infectious Diseases
Issue number5
Publication statusPublished - 1 Jan 1978
Externally publishedYes

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