MLST revisited: the gene-by-gene approach to bacterial genomics

Martin C J Maiden; Melissa J Jansen Van Rensburg; James E. Bray; Sarah G. Earle; Suzanne Ford; Keith A. Jolley; Noel D. McCarthy

doi:10.1038/nrmicro3093

MLST revisited: the gene-by-gene approach to bacterial genomics

Martin C J Maiden, Melissa J Jansen Van Rensburg, James E. Bray, Sarah G. Earle, Suzanne Ford, Keith A. Jolley, Noel D. McCarthy

Research output: Contribution to journal › Review Article › Research › peer-review

509 Citations (Scopus)

Abstract

Multilocus sequence typing (MLST) was proposed in 1998 as a portable sequence-based method for identifying clonal relationships among bacteria. Today, in the whole-genome era of microbiology, the need for systematic, standardized descriptions of bacterial genotypic variation remains a priority. Here, to meet this need, we draw on the successes of MLST and 16S rRNA gene sequencing to propose a hierarchical gene-by-gene approach that reflects functional and evolutionary relationships and catalogues bacteria 'from domain to strain'. Our gene-based typing approach using online platforms such as the Bacterial Isolate Genome Sequence Database (BIGSdb) allows the scalable organization and analysis of whole-genome sequence data.

Original language	English
Pages (from-to)	728-736
Number of pages	9
Journal	Nature Reviews Microbiology
Volume	11
Issue number	10
DOIs	https://doi.org/10.1038/nrmicro3093
Publication status	Published - Oct 2013
Externally published	Yes

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AU - Ford, Suzanne

AU - Jolley, Keith A.

AU - McCarthy, Noel D.

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MLST revisited: the gene-by-gene approach to bacterial genomics

Abstract

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