Abstract
Reef-building corals form associations with a huge diversity of microorganisms, which are essential for the survival and well-being of their host. While the acquisition patterns of Symbiodiniaceae microalgal endosymbionts are strongly linked to the coral's reproductive strategy, few studies have investigated the transmission mode of bacteria, especially in brooding species. Here, we relied on 16S rRNA gene and Internal Transcribed Spacer 2 marker metabarcoding in conjunction with fluorescence in situ hybridisation microscopy to describe the onset of microbial associations in the common brooding coral Pocillopora acuta. We analysed the bacterial and Symbiodiniaceae community composition in five adult colonies, their larvae, and 4-day old recruits. Larvae and recruits inherited Symbiodiniaceae, as well as a small number of bacterial strains, from their parents. Rhodobacteraceae and Endozoicomonas were among the most abundant taxa that were likely maternally transmitted to the offspring. The presence of bacterial aggregates in newly released larvae was observed with confocal microscopy, confirming the occurrence of vertical transmission of bacteria in P. acuta. We concluded that host factors, as well as the environmental bacterial pool influenced the microbiome of P. acuta.
| Original language | English |
|---|---|
| Pages (from-to) | 397-412 |
| Number of pages | 16 |
| Journal | Environmental Microbiology |
| Volume | 22 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2020 |
Cite this
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Mixed-mode bacterial transmission in the common brooding coral Pocillopora acuta. / Damjanovic, Katarina; Menéndez, Patricia; Blackall, Linda L.; van Oppen, Madeleine J. H.
In: Environmental Microbiology, Vol. 22, No. 1, 01.2020, p. 397-412.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Mixed-mode bacterial transmission in the common brooding coral Pocillopora acuta
AU - Damjanovic, Katarina
AU - Menéndez, Patricia
AU - Blackall, Linda L.
AU - van Oppen, Madeleine J. H.
PY - 2020/1
Y1 - 2020/1
N2 - Reef-building corals form associations with a huge diversity of microorganisms, which are essential for the survival and well-being of their host. While the acquisition patterns of Symbiodiniaceae microalgal endosymbionts are strongly linked to the coral's reproductive strategy, few studies have investigated the transmission mode of bacteria, especially in brooding species. Here, we relied on 16S rRNA gene and Internal Transcribed Spacer 2 marker metabarcoding in conjunction with fluorescence in situ hybridisation microscopy to describe the onset of microbial associations in the common brooding coral Pocillopora acuta. We analysed the bacterial and Symbiodiniaceae community composition in five adult colonies, their larvae, and 4-day old recruits. Larvae and recruits inherited Symbiodiniaceae, as well as a small number of bacterial strains, from their parents. Rhodobacteraceae and Endozoicomonas were among the most abundant taxa that were likely maternally transmitted to the offspring. The presence of bacterial aggregates in newly released larvae was observed with confocal microscopy, confirming the occurrence of vertical transmission of bacteria in P. acuta. We concluded that host factors, as well as the environmental bacterial pool influenced the microbiome of P. acuta.
AB - Reef-building corals form associations with a huge diversity of microorganisms, which are essential for the survival and well-being of their host. While the acquisition patterns of Symbiodiniaceae microalgal endosymbionts are strongly linked to the coral's reproductive strategy, few studies have investigated the transmission mode of bacteria, especially in brooding species. Here, we relied on 16S rRNA gene and Internal Transcribed Spacer 2 marker metabarcoding in conjunction with fluorescence in situ hybridisation microscopy to describe the onset of microbial associations in the common brooding coral Pocillopora acuta. We analysed the bacterial and Symbiodiniaceae community composition in five adult colonies, their larvae, and 4-day old recruits. Larvae and recruits inherited Symbiodiniaceae, as well as a small number of bacterial strains, from their parents. Rhodobacteraceae and Endozoicomonas were among the most abundant taxa that were likely maternally transmitted to the offspring. The presence of bacterial aggregates in newly released larvae was observed with confocal microscopy, confirming the occurrence of vertical transmission of bacteria in P. acuta. We concluded that host factors, as well as the environmental bacterial pool influenced the microbiome of P. acuta.
UR - http://www.scopus.com/inward/record.url?scp=85075480131&partnerID=8YFLogxK
U2 - 10.1111/1462-2920.14856
DO - 10.1111/1462-2920.14856
M3 - Article
C2 - 31709681
AN - SCOPUS:85075480131
VL - 22
SP - 397
EP - 412
JO - Environmental Microbiology
JF - Environmental Microbiology
SN - 1462-2912
IS - 1
ER -