Mitochondrial protein import: precursor oxidation in a ternary complex with disulfide carrier and sulfhydryl oxidase

Diana Stojanovski, Dusanka Milenkovic, Judith M Muller, Kipros Gabriel, Agnes Schulze-Specking, Michael J Baker, Martin T Ryan, Bernard Guiard, Nikolaus Pfanner, Agnieszka Chacinska

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67 Citations (Scopus)

Abstract

The biogenesis of mitochondrial intermembrane space proteins depends on specific machinery that transfers disulfide bonds to precursor proteins. The machinery shares features with protein relays for disulfide bond formation in the bacterial periplasm and endoplasmic reticulum. A disulfide-generating enzyme/sulfhydryl oxidase oxidizes a disulfide carrier protein, which in turn transfers a disulfide to the substrate protein. Current views suggest that the disulfide carrier alternates between binding to the oxidase and the substrate. We have analyzed the cooperation of the disulfide relay components during import of precursors into mitochondria and identified a ternary complex of all three components. The ternary complex represents a transient and intermediate step in the oxidation of intermembrane space precursors, where the oxidase Erv1 promotes disulfide transfer to the precursor while both oxidase and precursor are associated with the disulfide carrier Mia40.
Original languageEnglish
Pages (from-to)195 - 202
Number of pages8
JournalJournal of Cell Biology
Volume183
Issue number2
Publication statusPublished - 2008
Externally publishedYes

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