Mitochondrial imaging in live or fixed tissues using a luminescent iridium complex

Alexandra Sorvina, Christie A. Bader, Jack R.T. Darby, Mitchell C. Lock, Jia Yin Soo, Ian R.D. Johnson, Chiara Caporale, Nicolas H. Voelcker, Stefano Stagni, Massimiliano Massi, Janna L. Morrison, Sally E. Plush, Douglas A. Brooks

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3 Citations (Scopus)

Abstract

Mitochondrial morphology is important for the function of this critical organelle and, accordingly, altered mitochondrial structure is exhibited in many pathologies. Imaging of mitochondria can therefore provide important information about disease presence and progression. However, mitochondrial imaging is currently limited by the availability of agents that have the capacity to image mitochondrial morphology in both live and fixed samples. This can be particularly problematic in clinical studies or large, multi-centre cohort studies, where tissue archiving by fixation is often more practical. We previously reported the synthesis of an iridium coordination complex [Ir(ppy)2(MeTzPyPhCN)]+; where ppy is a cyclometalated 2-phenylpyridine and TzPyPhCN is the 5-(5-(4-cyanophen-1-yl)pyrid-2-yl)tetrazolate ligand; and showed that this complex (herein referred to as IraZolve-Mito) has a high specificity for mitochondria in live cells. Here we demonstrate that IraZolve-Mito can also effectively stain mitochondria in both live and fixed tissue samples. The staining protocol proposed is versatile, providing a universal procedure for cell biologists and pathologists to visualise mitochondria.

Original languageEnglish
Article number8191
Number of pages8
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 1 Dec 2018

Cite this

Sorvina, A., Bader, C. A., Darby, J. R. T., Lock, M. C., Soo, J. Y., Johnson, I. R. D., ... Brooks, D. A. (2018). Mitochondrial imaging in live or fixed tissues using a luminescent iridium complex. Scientific Reports, 8(1), [8191]. https://doi.org/10.1038/s41598-018-24672-w
Sorvina, Alexandra ; Bader, Christie A. ; Darby, Jack R.T. ; Lock, Mitchell C. ; Soo, Jia Yin ; Johnson, Ian R.D. ; Caporale, Chiara ; Voelcker, Nicolas H. ; Stagni, Stefano ; Massi, Massimiliano ; Morrison, Janna L. ; Plush, Sally E. ; Brooks, Douglas A. / Mitochondrial imaging in live or fixed tissues using a luminescent iridium complex. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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abstract = "Mitochondrial morphology is important for the function of this critical organelle and, accordingly, altered mitochondrial structure is exhibited in many pathologies. Imaging of mitochondria can therefore provide important information about disease presence and progression. However, mitochondrial imaging is currently limited by the availability of agents that have the capacity to image mitochondrial morphology in both live and fixed samples. This can be particularly problematic in clinical studies or large, multi-centre cohort studies, where tissue archiving by fixation is often more practical. We previously reported the synthesis of an iridium coordination complex [Ir(ppy)2(MeTzPyPhCN)]+; where ppy is a cyclometalated 2-phenylpyridine and TzPyPhCN is the 5-(5-(4-cyanophen-1-yl)pyrid-2-yl)tetrazolate ligand; and showed that this complex (herein referred to as IraZolve-Mito) has a high specificity for mitochondria in live cells. Here we demonstrate that IraZolve-Mito can also effectively stain mitochondria in both live and fixed tissue samples. The staining protocol proposed is versatile, providing a universal procedure for cell biologists and pathologists to visualise mitochondria.",
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Sorvina, A, Bader, CA, Darby, JRT, Lock, MC, Soo, JY, Johnson, IRD, Caporale, C, Voelcker, NH, Stagni, S, Massi, M, Morrison, JL, Plush, SE & Brooks, DA 2018, 'Mitochondrial imaging in live or fixed tissues using a luminescent iridium complex', Scientific Reports, vol. 8, no. 1, 8191. https://doi.org/10.1038/s41598-018-24672-w

Mitochondrial imaging in live or fixed tissues using a luminescent iridium complex. / Sorvina, Alexandra; Bader, Christie A.; Darby, Jack R.T.; Lock, Mitchell C.; Soo, Jia Yin; Johnson, Ian R.D.; Caporale, Chiara; Voelcker, Nicolas H.; Stagni, Stefano; Massi, Massimiliano; Morrison, Janna L.; Plush, Sally E.; Brooks, Douglas A.

In: Scientific Reports, Vol. 8, No. 1, 8191, 01.12.2018.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Sorvina, Alexandra

AU - Bader, Christie A.

AU - Darby, Jack R.T.

AU - Lock, Mitchell C.

AU - Soo, Jia Yin

AU - Johnson, Ian R.D.

AU - Caporale, Chiara

AU - Voelcker, Nicolas H.

AU - Stagni, Stefano

AU - Massi, Massimiliano

AU - Morrison, Janna L.

AU - Plush, Sally E.

AU - Brooks, Douglas A.

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JF - Scientific Reports

SN - 2045-2322

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Sorvina A, Bader CA, Darby JRT, Lock MC, Soo JY, Johnson IRD et al. Mitochondrial imaging in live or fixed tissues using a luminescent iridium complex. Scientific Reports. 2018 Dec 1;8(1). 8191. https://doi.org/10.1038/s41598-018-24672-w