miRNA malfunction causes spinal motor neuron disease

Sharon Haramati, Elik Chapnik, Yehezkel Sztainberg, Raya Eilam, Raaya Zwang, Noga Gershoni, Edwina McGlinn, Patrick Heiser, Anne-Marie Wills, Itzhak Wirguin, Lee Rubin, Hidemi Misawa, Clifford Tabin, Robert Brown Jr., Alon Chen, Eran Hornstein

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187 Citations (Scopus)

Abstract

Defective RNA metabolism is an emerging mechanism involved in ALS pathogenesis and possibly in other neurodegenerative disorders. Here, we show that microRNA (miRNA) activity is essential for long-term survival of postmitotic spinal motor neurons (SMNs) in vivo. Thus, mice that do not process miRNA in SMNs exhibit hallmarks of spinal muscular atrophy (SMA), including sclerosis of the spinal cord ventral horns, aberrant end plate architecture, and myofiber atrophy with signs of denervation. Furthermore, a neurofilament heavy subunit previously implicated in motor neuron degeneration is specifically up-regulated in miRNA-deficient SMNs. We demonstrate that the heavy neurofilament subunit is a target of miR-9, a miRNA that is specifically down-regulated in a genetic model of SMA. These data provide evidence for miRNA function in SMN diseases and emphasize the potential role of miR-9-based regulatory mechanisms in adult neurons and neurodegenerative states.
Original languageEnglish
Pages (from-to)13111 - 13116
Number of pages6
JournalProceedings of the National Academy of Sciences
Volume107
Issue number29
DOIs
Publication statusPublished - 2010
Externally publishedYes

Cite this

Haramati, S., Chapnik, E., Sztainberg, Y., Eilam, R., Zwang, R., Gershoni, N., ... Hornstein, E. (2010). miRNA malfunction causes spinal motor neuron disease. Proceedings of the National Academy of Sciences, 107(29), 13111 - 13116. https://doi.org/10.1073/pnas.1006151107/-/DCSupplemental
Haramati, Sharon ; Chapnik, Elik ; Sztainberg, Yehezkel ; Eilam, Raya ; Zwang, Raaya ; Gershoni, Noga ; McGlinn, Edwina ; Heiser, Patrick ; Wills, Anne-Marie ; Wirguin, Itzhak ; Rubin, Lee ; Misawa, Hidemi ; Tabin, Clifford ; Brown Jr., Robert ; Chen, Alon ; Hornstein, Eran. / miRNA malfunction causes spinal motor neuron disease. In: Proceedings of the National Academy of Sciences. 2010 ; Vol. 107, No. 29. pp. 13111 - 13116.
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title = "miRNA malfunction causes spinal motor neuron disease",
abstract = "Defective RNA metabolism is an emerging mechanism involved in ALS pathogenesis and possibly in other neurodegenerative disorders. Here, we show that microRNA (miRNA) activity is essential for long-term survival of postmitotic spinal motor neurons (SMNs) in vivo. Thus, mice that do not process miRNA in SMNs exhibit hallmarks of spinal muscular atrophy (SMA), including sclerosis of the spinal cord ventral horns, aberrant end plate architecture, and myofiber atrophy with signs of denervation. Furthermore, a neurofilament heavy subunit previously implicated in motor neuron degeneration is specifically up-regulated in miRNA-deficient SMNs. We demonstrate that the heavy neurofilament subunit is a target of miR-9, a miRNA that is specifically down-regulated in a genetic model of SMA. These data provide evidence for miRNA function in SMN diseases and emphasize the potential role of miR-9-based regulatory mechanisms in adult neurons and neurodegenerative states.",
author = "Sharon Haramati and Elik Chapnik and Yehezkel Sztainberg and Raya Eilam and Raaya Zwang and Noga Gershoni and Edwina McGlinn and Patrick Heiser and Anne-Marie Wills and Itzhak Wirguin and Lee Rubin and Hidemi Misawa and Clifford Tabin and {Brown Jr.}, Robert and Alon Chen and Eran Hornstein",
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Haramati, S, Chapnik, E, Sztainberg, Y, Eilam, R, Zwang, R, Gershoni, N, McGlinn, E, Heiser, P, Wills, A-M, Wirguin, I, Rubin, L, Misawa, H, Tabin, C, Brown Jr., R, Chen, A & Hornstein, E 2010, 'miRNA malfunction causes spinal motor neuron disease', Proceedings of the National Academy of Sciences, vol. 107, no. 29, pp. 13111 - 13116. https://doi.org/10.1073/pnas.1006151107/-/DCSupplemental

miRNA malfunction causes spinal motor neuron disease. / Haramati, Sharon; Chapnik, Elik; Sztainberg, Yehezkel; Eilam, Raya; Zwang, Raaya; Gershoni, Noga; McGlinn, Edwina; Heiser, Patrick; Wills, Anne-Marie; Wirguin, Itzhak; Rubin, Lee; Misawa, Hidemi; Tabin, Clifford; Brown Jr., Robert; Chen, Alon; Hornstein, Eran.

In: Proceedings of the National Academy of Sciences, Vol. 107, No. 29, 2010, p. 13111 - 13116.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Haramati, Sharon

AU - Chapnik, Elik

AU - Sztainberg, Yehezkel

AU - Eilam, Raya

AU - Zwang, Raaya

AU - Gershoni, Noga

AU - McGlinn, Edwina

AU - Heiser, Patrick

AU - Wills, Anne-Marie

AU - Wirguin, Itzhak

AU - Rubin, Lee

AU - Misawa, Hidemi

AU - Tabin, Clifford

AU - Brown Jr., Robert

AU - Chen, Alon

AU - Hornstein, Eran

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AB - Defective RNA metabolism is an emerging mechanism involved in ALS pathogenesis and possibly in other neurodegenerative disorders. Here, we show that microRNA (miRNA) activity is essential for long-term survival of postmitotic spinal motor neurons (SMNs) in vivo. Thus, mice that do not process miRNA in SMNs exhibit hallmarks of spinal muscular atrophy (SMA), including sclerosis of the spinal cord ventral horns, aberrant end plate architecture, and myofiber atrophy with signs of denervation. Furthermore, a neurofilament heavy subunit previously implicated in motor neuron degeneration is specifically up-regulated in miRNA-deficient SMNs. We demonstrate that the heavy neurofilament subunit is a target of miR-9, a miRNA that is specifically down-regulated in a genetic model of SMA. These data provide evidence for miRNA function in SMN diseases and emphasize the potential role of miR-9-based regulatory mechanisms in adult neurons and neurodegenerative states.

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JF - Proceedings of the National Academy of Sciences of the United States of America

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Haramati S, Chapnik E, Sztainberg Y, Eilam R, Zwang R, Gershoni N et al. miRNA malfunction causes spinal motor neuron disease. Proceedings of the National Academy of Sciences. 2010;107(29):13111 - 13116. https://doi.org/10.1073/pnas.1006151107/-/DCSupplemental