Minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE)

Eric Deutsch, Catherine Ball, Jules Berman, Steven G Bova, Alvis Brazma, Roger Bumgarner, David Campbell, Helen Causton, Jeffrey Christiansen, Fabrice Daian, Delphone Dauga, Duncan Davidson, Gregory Gimenez, Young Goo, Sean Grimmond, Thorsten Henrich, Bernhard Herrmann, Michael Johnson, Martin Korb, Jason MillsAsa Oudes, Helen Parkinson, Laura Pascal, Nicolas Pollet, John Quackenbush, Mirana Ramialison, Martin Ringwald, David Salgado, Susanna-Assunta Sansone, Gavin Sherlock, Christian Stoeckert Jnr, Jason Swedlow, Ronald Taylor, Laura Walashek, Anthony Warford, David Wilkinson, Yi Zhou, Leonard I Zon, Alvin Liu, Lawrence True

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Abstract

One purpose of the biomedical literature is to report results in sufficient detail that the methods of data collection and analysis can be independently replicated and verified. Here we present reporting guidelines for gene expression localization experiments: the minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE). MISFISHIE is modeled after the Minimum Information About a Microarray Experiment (MIAME) specification for microarray experiments. Both guidelines define what information should be reported without dictating a format for encoding that information. MISFISHIE describes six types of information to be provided for each experiment: experimental design, biomaterials and treatments, reporters, staining, imaging data and image characterizations. This specification has benefited the consortium within which it was developed and is expected to benefit the wider research community. We welcome feedback from the scientific community to help improve our proposal.
Original languageEnglish
Pages (from-to)305 - 312
Number of pages8
JournalNature Biotechnology
Volume26
Issue number3
DOIs
Publication statusPublished - 2008
Externally publishedYes

Cite this

Deutsch, E., Ball, C., Berman, J., Bova, S. G., Brazma, A., Bumgarner, R., ... True, L. (2008). Minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE). Nature Biotechnology, 26(3), 305 - 312. https://doi.org/10.1038/nbt1391