Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system

Angel Alberto Jesus Torriero, Eloy Salinas, Fernando Battaglini, Julio Raba

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17 Citations (Scopus)

Abstract

The high sensitivity that can be attained using a bienzymic system and mediated by the redox polymer [Os(bpy)2ClPyCH2NHpoly(allylamine)] (Os-PAA), has been verified by online interfacing of a rotating bioreactor and continuous-flow/stopped-flow/continuous-flow processing. When the hydrogen peroxide formed by LOx layer reaches the inner layer, the electronic flow between the immobilized peroxidase and the electrode surface produces a current, proportional to lactate concn. The detn. of lactate was possible with a limit of detection of 5 nmol/L in the processing of as many as 30 samples per h. This arrangement allows working in undiluted milk samples with a good stability and reproducibility. Horseradish peroxidase [EC 1.11.1.7] and Os-PAA were covalently immobilized on the glassy carbon electrode surface (upper cell body), lactate oxidase [EC 1.1.3.x] was immobilized on a disk that can be rotated. [on SciFinder (R)]
Original languageEnglish
Pages (from-to)155 - 163
Number of pages9
JournalAnalytica Chimica Acta
Volume498
Issue number1-2
Publication statusPublished - 2003
Externally publishedYes

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