Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system

Angel Alberto Jesus Torriero, Eloy Salinas, Fernando Battaglini, Julio Raba

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)


The high sensitivity that can be attained using a bienzymic system and mediated by the redox polymer [Os(bpy)2ClPyCH2NHpoly(allylamine)] (Os-PAA), has been verified by online interfacing of a rotating bioreactor and continuous-flow/stopped-flow/continuous-flow processing. When the hydrogen peroxide formed by LOx layer reaches the inner layer, the electronic flow between the immobilized peroxidase and the electrode surface produces a current, proportional to lactate concn. The detn. of lactate was possible with a limit of detection of 5 nmol/L in the processing of as many as 30 samples per h. This arrangement allows working in undiluted milk samples with a good stability and reproducibility. Horseradish peroxidase [EC] and Os-PAA were covalently immobilized on the glassy carbon electrode surface (upper cell body), lactate oxidase [EC 1.1.3.x] was immobilized on a disk that can be rotated. [on SciFinder (R)]
Original languageEnglish
Pages (from-to)155 - 163
Number of pages9
JournalAnalytica Chimica Acta
Issue number1-2
Publication statusPublished - 2003
Externally publishedYes

Cite this